食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
1期
109-113
,共5页
李丽%熊思驰%黄飞%王国盼%韦春葵%苏宏飞%梁智群%黄时海
李麗%熊思馳%黃飛%王國盼%韋春葵%囌宏飛%樑智群%黃時海
리려%웅사치%황비%왕국반%위춘규%소굉비%량지군%황시해
海洋红酵母%L-苯丙氨酸解氨酶%原生质体诱变
海洋紅酵母%L-苯丙氨痠解氨酶%原生質體誘變
해양홍효모%L-분병안산해안매%원생질체유변
Rhodotorula benthica%L-phenylalanine ammonia-lyase%protoplast mutation
原始菌株海洋红酵母M1202产生的L-苯丙氨酸解氨酶（PAL），能够逆反应催化反式肉桂酸（t-Ca）生成L-苯丙氨酸。对M1202进行原生质体紫外诱变获得一株转化率为原始菌株115%的菌株TM2，经6次传代，仍具有较好的遗传稳定性。
原始菌株海洋紅酵母M1202產生的L-苯丙氨痠解氨酶（PAL），能夠逆反應催化反式肉桂痠（t-Ca）生成L-苯丙氨痠。對M1202進行原生質體紫外誘變穫得一株轉化率為原始菌株115%的菌株TM2，經6次傳代，仍具有較好的遺傳穩定性。
원시균주해양홍효모M1202산생적L-분병안산해안매（PAL），능구역반응최화반식육계산（t-Ca）생성L-분병안산。대M1202진행원생질체자외유변획득일주전화솔위원시균주115%적균주TM2，경6차전대，잉구유교호적유전은정성。
Using Rhodotorula benthica M1202 as the original strain, the L-phenylalanine ammonia-lyase (PAL) of M1202 could transform trans-Cinnamic acid (t-Ca) to L-phenylalanine. The aim of this study was to obtain new Rhodotorula benthica mutants with high conversion rate. UV induced mutagenesis of protoplast was performed on original strain Rhodotorula benthica M1202. A genetically stable mutant strains TM2 was selected from a large amount of the regenerative mutants. The conversion rate of TM2 was 1.15-fold increased compared with its original strain. Further experiment confirmed after six generations successively propagating the conversion rate of TM2 was stable.
