食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
1期
99-102
,共4页
硒%催化光度法%表面活性剂
硒%催化光度法%錶麵活性劑
서%최화광도법%표면활성제
selenium%catalytic spectrophotometry%surface active agent
讨论利用硒催化溴酸钾氧化甲基橙褪色的原理建立了一种测定硒的方法，确定在测定波长λ为506 nm，硒浓度在0.2μg/mL～1.2μg/mL范围内与△A呈良好的线性关系，测定的线性回归方程为△A=0.1814C+0.107，相关系数γ＝0.9979，表观摩尔吸光系数为2.94×104 L·mol-1·cm-1。测定富硒盐中含硒量为4.275 mg/kg，加标回收率在93.3%～96.4%之间，并且RSD为1.2%，实验结果比较令人满意，可作为快速测定食品中的痕量硒的方法。
討論利用硒催化溴痠鉀氧化甲基橙褪色的原理建立瞭一種測定硒的方法，確定在測定波長λ為506 nm，硒濃度在0.2μg/mL～1.2μg/mL範圍內與△A呈良好的線性關繫，測定的線性迴歸方程為△A=0.1814C+0.107，相關繫數γ＝0.9979，錶觀摩爾吸光繫數為2.94×104 L·mol-1·cm-1。測定富硒鹽中含硒量為4.275 mg/kg，加標迴收率在93.3%～96.4%之間，併且RSD為1.2%，實驗結果比較令人滿意，可作為快速測定食品中的痕量硒的方法。
토론이용서최화추산갑양화갑기등퇴색적원리건립료일충측정서적방법，학정재측정파장λ위506 nm，서농도재0.2μg/mL～1.2μg/mL범위내여△A정량호적선성관계，측정적선성회귀방정위△A=0.1814C+0.107，상관계수γ＝0.9979，표관마이흡광계수위2.94×104 L·mol-1·cm-1。측정부서염중함서량위4.275 mg/kg，가표회수솔재93.3%～96.4%지간，병차RSD위1.2%，실험결과비교령인만의，가작위쾌속측정식품중적흔량서적방법。
In this thesis, a method of determination selenium based on the color fading of methyl orange with potassium bromate was catalyzed by cupricion. With the detection wavelengthλwas 506 nm ,linear relationship between the magnitude of decrease in absorbance△A and mass concentration of selenium was obtained in the range of 0.2μg/mL-1.2μg/mL. The linear regression equation△A=0.181 4C+0.107, correlation coefficientγ=0.997 9 and the apparent molar absorptivity of 2.94 × 104 L·mol-1·cm-1. Values of recovery found by standard addition method were in the range from 93.3%to 96.4%, with values of RSD (n=5) was 1.2%.
