CAJ | 학술논문

利用超声耦合乙醇-硫酸铵双水相萃取技术提取牛大力总黄酮。通过单因素实验和正交实验对影响牛大力总黄酮提取的料液比、硫酸铵浓度、乙醇浓度和超声时间等因素进行探讨,以建立牛大力总黄酮的提取工艺。结果显示：影响牛大力总黄酮提取因素的主次顺序为料液比＞乙醇浓度＞硫酸铵浓度＞超声时间；最优提取条件为乙醇浓度80%、料液比1∶40(m∶V )、超声时间50 min、硫酸铵浓度为0.3 g/mL,在此条件下,牛大力总黄酮得率为5.82 mg/g。
이용초성우합을순-류산안쌍수상췌취기술제취우대력총황동。통과단인소실험화정교실험대영향우대력총황동제취적료액비、류산안농도、을순농도화초성시간등인소진행탐토,이건립우대력총황동적제취공예。결과현시：영향우대력총황동제취인소적주차순서위료액비＞을순농도＞류산안농도＞초성시간；최우제취조건위을순농도80%、료액비1∶40(m∶V )、초성시간50 min、류산안농도위0.3 g/mL,재차조건하,우대력총황동득솔위5.82 mg/g。
In this study, flavonoids from Millettia speciosa roots was extracted by ultrasonic-assisted alcohol-ammonium sulphate aqueous two-phase system. To determine the optimal extraction conditions, the effect of the conditions on yield, such as the ethanol concentration, the solid-liquid ratio, the amount of the ammonium sulfate and the ultrasonic time were studied by single factor experiment and orthogonal test. The results showed that the order of factor affecting total flavones extraction was solid-liquid ratio >ethanol concentration > ammonium sulfate concentration > ultrasonic time. The optimum extracting conditions were as follows: the volume fraction of alcohol 80%, solid to solution ratio of 1:40, ultrasonic time 50 min, the ammonium sulfate 0.3 g/mL. At the optimum conditions, the extraction rate of the flavonoids could reach 5.82 mg/g.