CAJ | 학술논문

研制了海藻糖国家标准样品。以食品级海藻糖粗品为原料，纯化制备海藻糖，采用红外光谱(IR)、质谱和核磁共振谱(NMR)以及单晶衍射等方法进行结构确证。样品分装成400瓶后，采用离子色谱法进行均匀性、稳定性检验和定值分析。从样品中随机抽取15瓶进行均匀性检验，经F检验表明，在95%的置信区间范围内，样品均匀性良好。在40℃下，经过24个月稳定性考察，结果表明样品稳定性良好。标准样品经国内8家具有分析资质的实验室进行协同定值，并评定了定值结果的不确定度，海藻糖标准样品定值结果为99.72%，扩展不确定度为0.26%(k=1.96)。该标准样品达到国家标准样品的技术要求，可用于有关海藻糖的方法校正和质量控制。
연제료해조당국가표준양품。이식품급해조당조품위원료，순화제비해조당，채용홍외광보(IR)、질보화핵자공진보(NMR)이급단정연사등방법진행결구학증。양품분장성400병후，채용리자색보법진행균균성、은정성검험화정치분석。종양품중수궤추취15병진행균균성검험，경F검험표명，재95%적치신구간범위내，양품균균성량호。재40℃하，경과24개월은정성고찰，결과표명양품은정성량호。표준양품경국내8가구유분석자질적실험실진행협동정치，병평정료정치결과적불학정도，해조당표준양품정치결과위99.72%，확전불학정도위0.26%(k=1.96)。해표준양품체도국가표준양품적기술요구，가용우유관해조당적방법교정화질량공제。
Trehalose certified reference material was developed.Trehalose was purified from the crude product. The structure of trehalose certified reference material was comfirmed by infrared spectroscopy (IR),mass spectrometry, NRM and XRD.Trehalose was divided into 400 bottles.The homogeneity and stability testing and quantitative analysis were evaluated by ion chromatography method.According to analysis procedure of homogeneity,15 bottles of sample were randomly taken from 400 bottles,and the results were validated by F-test statistical methods.The stability inspec-tion was carried on the long-term(24 months),and the results indicated that the period for trehalose storage was 24 months at 40℃.A cooperative certification was conducted by 8 qualified laboratories.The certified purity value of the reference material of trehalose was 99.72%with relative expaned uncertainty of 0.26%(k=1.96).The reference material can conform to the technical requirement of the certified reference material,and it can be used for validation and quality control regarding trehalose.