CAJ | 학술논문

目的：探讨6个微小 RNA （ microRNA ）多态性位点（ rs11614913， microRNA－196a2；rs3746444， hsa－mir－499；rs2910164，microRNA－146a；rs2292832，microRNA－149；rs6505162，microRNA－423；rs895819，microRNA－27a）与中国女性乳腺癌发病风险及病理特征的相关性。方法采用病例对照研究，选取350例乳腺癌患者为病例组及350名正常女性为对照组，采用Massarray SNP技术检测6个多态性位点的基因型，采用免疫组化法检测乳腺癌组织中的雌激素受体及孕激素受体表达，分析各基因型与乳腺癌发生的关系及与乳腺癌病理参数的关系。结果 rs3746444GG基因型在病例组中（OR＝1．71，95％CI：1．16～2．52）的频率显著高于对照组。分层分析结果显示，在绝经后的人群中，rs3746444GG基因型病例组中的频率（ OR＝2．19，95％CI：1．16～4．15）显著高于对照组。 rs3746444GG基因型在肿瘤分期0～Ⅱ组（OR＝1．92，95％CI：1．17～3．13）、淋巴结转移组（OR＝2．20，95％CI：1．28～3．79）、孕激素受体阴性组中（OR＝2．19，95％CI：1．25～3．82）分布频率均显著高于正常对照组。在绝经后人群中，rs2910164GG基因型在病例组中（OR＝2．19，95％CI：1．16～4．15）的分布频率显著高于对照组。而在绝经前的人群中，rs2910164GG基因型在病例组中（ OR＝0．49，95％CI：0．25～0．98）的分布频率显著低于对照组。结论rs3746444GG基因型与乳腺癌发病风险相关，特别是在绝经后人群中风险更高。 rs2910164GG基因型与乳腺癌的发病风险相关性与妇女是否绝经有关。
목적：탐토6개미소 RNA （ microRNA ）다태성위점（ rs11614913， microRNA－196a2；rs3746444， hsa－mir－499；rs2910164，microRNA－146a；rs2292832，microRNA－149；rs6505162，microRNA－423；rs895819，microRNA－27a）여중국녀성유선암발병풍험급병리특정적상관성。방법채용병례대조연구，선취350례유선암환자위병례조급350명정상녀성위대조조，채용Massarray SNP기술검측6개다태성위점적기인형，채용면역조화법검측유선암조직중적자격소수체급잉격소수체표체，분석각기인형여유선암발생적관계급여유선암병리삼수적관계。결과 rs3746444GG기인형재병례조중（OR＝1．71，95％CI：1．16～2．52）적빈솔현저고우대조조。분층분석결과현시，재절경후적인군중，rs3746444GG기인형병례조중적빈솔（ OR＝2．19，95％CI：1．16～4．15）현저고우대조조。 rs3746444GG기인형재종류분기0～Ⅱ조（OR＝1．92，95％CI：1．17～3．13）、림파결전이조（OR＝2．20，95％CI：1．28～3．79）、잉격소수체음성조중（OR＝2．19，95％CI：1．25～3．82）분포빈솔균현저고우정상대조조。재절경후인군중，rs2910164GG기인형재병례조중（OR＝2．19，95％CI：1．16～4．15）적분포빈솔현저고우대조조。이재절경전적인군중，rs2910164GG기인형재병례조중（ OR＝0．49，95％CI：0．25～0．98）적분포빈솔현저저우대조조。결론rs3746444GG기인형여유선암발병풍험상관，특별시재절경후인군중풍험경고。 rs2910164GG기인형여유선암적발병풍험상관성여부녀시부절경유관。
Objective To investigate the relationship between the polymorphisms in microRNAs ( rs11614913, miRNA-196a2;rs3746444, hsa-mir-499;rs2910164, miRNA-146a;rs2292832, miRNA-149;rs6505162, miRNA-423;rs895819, miRNA-27a) and risk and pathologic characterize of breast cancer in Chinese female population .Methods A total of 350 patients with breast cancer and 350 health controls were enrolled for this case-controls study .Sequenom MassARRAY was applied to detect the polymor-phisms, and the immunohistochemistry assay was used to measure the expression of estrogen receptor (ER) and progesterone receptor ( PR) .Logistic regression was applied to assess the association between the polymorphisms and risk and pathologic characterize of breast cancer.Results The results showed that the frequency of 3746444GG genotype(OR=1.71, 95%CI:1.16-2.52) was high-er in cases than that in health controls .Sub-group analysis revealed that , in the subgroup of women with postmenopausal status , the frequency of 3746444GG genotype (OR=2.19, 95%CI:1.16-4.15) was higher in cases than that in health controls .Moreover, the frequency of 3746444GG was higher in subgroup of patients with tumor stage 0-Ⅱ(OR=1.92, 95%CI:1.17-3.13), lymph node involvement(OR=2.20, 95%CI:1.28-3.79), negative PR expression (OR=2.19, 95% CI:1.25-3.82) than that in health controls, respectively.In addition, rs2910164GG genotype(OR=2.19, 95%CI:1.16-4.15) was associated with increased breast cancer risk in the postmenopausal status sub-cohort, while, rs2910164GG genotype (OR=0.49, 95%CI:0.25-0.98) was associated with decreased breast cancer risk in the premenopausal status sub -cohort.Conclusion rs3746444GG genotype was associ-ated with breast cancer risk , especiallyfor women who with postmenopausal status .The risk of rs2910164GG for breast cancer was mod-ified by the postmenopausal status .