CAJ | 학술논문

为研究自然放牧乌珠穆沁羊成长过程中骨骼肌内M STN基因及其蛋白表达量的变化。分别采用实时荧光定量和ELISA技术测定乌珠穆沁羊生长过程中骨骼肌MSTN基因相对表达量和蛋白表达量。 MSTN基因相对表达量为6月龄＞3月龄＞9月龄＞1月龄＞12月龄＞18月龄，6月龄的基因表达量最高，与其他月龄之间相比有显著差异（P＜0．05）。1，3，9，12月龄之间基因表达量无显著差异（P＞0．05）。 MSTN蛋白表达量为1月龄＜3月龄＜6月龄＜9月龄＜12月龄＜18月龄，1，3，6月龄的表达量之间有显著差异（ P＜0．05），9，12，18月龄的表达量之间无显著差异（ P＞0．05）。 MSTN基因相对表达水平1～6月龄之间有上升趋势之后又下降。 MSTN蛋白表达水平一直为上升趋势。
위연구자연방목오주목심양성장과정중골격기내M STN기인급기단백표체량적변화。분별채용실시형광정량화ELISA기술측정오주목심양생장과정중골격기MSTN기인상대표체량화단백표체량。 MSTN기인상대표체량위6월령＞3월령＞9월령＞1월령＞12월령＞18월령，6월령적기인표체량최고，여기타월령지간상비유현저차이（P＜0．05）。1，3，9，12월령지간기인표체량무현저차이（P＞0．05）。 MSTN단백표체량위1월령＜3월령＜6월령＜9월령＜12월령＜18월령，1，3，6월령적표체량지간유현저차이（ P＜0．05），9，12，18월령적표체량지간무현저차이（ P＞0．05）。 MSTN기인상대표체수평1～6월령지간유상승추세지후우하강。 MSTN단백표체수평일직위상승추세。
The Myostatin( MSTN) gene and protein expressions in the skeletal muscle of Ujumqin Sheep were studied during its growth .RT-PCR and ELISA were applied to detect the relative expression level of MSTN gene and protein,respectively,in the skeletal muscle of Ujumqin sheep at the age of 1,3,6,9,12 and 18 months.The relative MSTN gene expression level was in the descending sequence of 6>3>9>1>12>18-month old Ujumqin sheep. The MSTN gene expression was highest at the age of 6-month,and was significantly different from that of 1,3,9,12 and 18-month (P<0.05).The detected MSTN gene expression was not significantly different among samples col-lected from 1,3,9 and 12-month old sheep(P>0.05).The protein expression level was in the ascending sequence of 1<3<6<9<12<18-month old sheep.Samples from 1,3 and6-month old (P<0.05),but not 9,12 and 18 (P>0.05),sheep were significantly varied.The relative gene expression level of MSTN showed an increasing trend before 6-month old and decreased afterwards .The expression level of MSTN protein was increasing during the whole period .