CAJ | 학술논문

TALEN（ Transcription activator-like effector nuclease ）和CRISPR／Cas系统（ Clustered regularly interspaced short palindromic repeats，CRISPR．CRISPR-associated，Cas）是近年来发现的基因功能研究的新方法，且已经成功地在动物、植物及微生物等多个领域得到成功应用。它们可以对DNA双链进行切割，从而启动细胞内的DNA修复机制，以此来实现对靶位点的基因修饰。相较于传统方法，它们具有特异性高、操作简便及打靶效率高等特点。主要从结构、功能及作用机制等方面阐述这2种新基因功能的研究方法。
TALEN（ Transcription activator-like effector nuclease ）화CRISPR／Cas계통（ Clustered regularly interspaced short palindromic repeats，CRISPR．CRISPR-associated，Cas）시근년래발현적기인공능연구적신방법，차이경성공지재동물、식물급미생물등다개영역득도성공응용。타문가이대DNA쌍련진행절할，종이계동세포내적DNA수복궤제，이차래실현대파위점적기인수식。상교우전통방법，타문구유특이성고、조작간편급타파효솔고등특점。주요종결구、공능급작용궤제등방면천술저2충신기인공능적연구방법。
TALEN( Transcription activator-like effector nuclease ) CRISPR/Cas system ( Clustered regularly in-terspaced short palindromic repeats ,CRISPR.CRISPR-associated,Cas) have recently emerged as new technique for targeted genome modifications .They have been successfully applied in many fields such as animals ,plants and mi-croorganism .They can generate a double-strand break ( DSB ) .Repair of these DSBs by intracellular DNA repair mechanisms enables to achieve the target gene modification .Compared with traditional method , TALEN and CRISPR/Cas system were stronger specificity ,manipulated more easily and higher targeting efficiency .Here we re-port the construction ,function and mechanism of the TALEN and CRISPR/Cas system .