CAJ | 학술논문

为探索干热处理后带菌种子贮藏时间对种子发芽率及病菌活力的影响，明确干热处理后种子贮藏的最佳条件。以3种自然带菌的黄瓜种子为材料，将不同干热处理后的带菌黄瓜种子在4℃中分别贮藏1，2，3，6，9个月，测定贮藏时间对种子发芽率的影响，同时采用平板保湿培养法和荧光双染技术测定种子上病原真菌活力的变化。结果表明，干热处理带菌黄瓜种子的发芽率和种子上病原菌的活力随贮藏时间的延长呈现下降趋势。70℃处理40，60 min的黄瓜种子贮藏3个月后，3种带菌种子的发芽率均保持在88％以上，贮藏6个月后，发芽率均下降至约80％，70℃处理90 min的黄瓜种子贮藏3个月后，3种带菌种子的发芽率均下降至75％以下；而贮藏3个月后，带菌种子内外均无病原菌生长，孢子死亡率均上升至100％。说明70℃干热处理40，60 min后的带菌黄瓜种子贮藏3个月后发芽率仍在88％以上且病原菌死亡率达100％，因此，可作为种子贮藏前的处理条件，但70℃干热处理90 min后的种子适宜直接播种，且干热处理后的种子不适宜长期贮藏。
위탐색간열처리후대균충자저장시간대충자발아솔급병균활력적영향，명학간열처리후충자저장적최가조건。이3충자연대균적황과충자위재료，장불동간열처리후적대균황과충자재4℃중분별저장1，2，3，6，9개월，측정저장시간대충자발아솔적영향，동시채용평판보습배양법화형광쌍염기술측정충자상병원진균활력적변화。결과표명，간열처리대균황과충자적발아솔화충자상병원균적활력수저장시간적연장정현하강추세。70℃처리40，60 min적황과충자저장3개월후，3충대균충자적발아솔균보지재88％이상，저장6개월후，발아솔균하강지약80％，70℃처리90 min적황과충자저장3개월후，3충대균충자적발아솔균하강지75％이하；이저장3개월후，대균충자내외균무병원균생장，포자사망솔균상승지100％。설명70℃간열처리40，60 min후적대균황과충자저장3개월후발아솔잉재88％이상차병원균사망솔체100％，인차，가작위충자저장전적처리조건，단70℃간열처리90 min후적충자괄의직접파충，차간열처리후적충자불괄의장기저장。
In order to explore the effect of storage time on germination rate of infected seeds treated with dry heat and the activity of pathogenic fungi on infected seeds ,and figure out the best condition for storage .In this paper ,we used three different infected cucumber seeds as materials .The seeds were storing in 4 ℃for 1,2,3,6,9 months re-spectively after treated with dry heat .The seed was evaluated by determining seed germination rate ,and the pathogenic activities in the seeds were also evaluated by tablet moisturizing culture method as well as double fluorescence stai -ning.The results indicated that the seed germination rate and the vigor of pathogenic fungi on infected seeds were re -duced with the extending of storage time after stored 1,2,3,6,9 months respectively .Seed germination rate was main-tain above 88%after 3 months storage and about 80%after 6 months storage when the infected seeds treated 40,60 min under 70℃respectively .However ,seed germination rate was below 75%after 3 months storage when the infected seeds treated 90 min under 70℃.There was no pathogenic fungi detected and the mortality of the spores on the sur -face or internal of the infected seed treated with dry heat after stored 3 months.The experiment suggested that the seeds germination rate and the vigor of pathogenic fungi on infected seeds were decreased after storing the dry heat treated infected seeds .When the infected seeds treated 40,60 min under 70 ℃ seeds,germination rate were above 90%and the pathogen had lost its vitality .Therefore these can be used as treatment condition before seed storage .