CAJ | 학술논문

通过对甘薯软腐病菌侵染甘薯的发病特征进行研究，首次建立了一种甘薯软腐病抗性鉴定的方法：每个品种取3块中等大小的薯块，经严格消毒晾干后，切薯块中部薯片，每薯块切3个薯片，每薯片厚度约为8 mm，置于放有灭菌湿润滤纸保湿的灭菌培养皿中，接种1块6 mm的甘薯软腐病菌菌碟于薯片中央，将接种好的薯片置于26℃生化培养箱中培养，期间加无菌水保湿1次。21 h后将鉴定材料取出，量取薯片发病直径，根据病斑直径进行分级，计算病情指数，进行抗感评价。该方法的建立为抗病品种的选育提供了理论依据。利用该方法对44份品种资源进行抗性评价，结果显示，鲁薯4号和广紫8号对甘薯软腐病的抗性级别为中抗；湛薯271、泰中11、渝紫1号、赣薯1号和PZJ商27 E2-2对甘薯软腐病的抗性级别为感病；其余品种对甘薯软腐病的抗性级别均为高感。
통과대감서연부병균침염감서적발병특정진행연구，수차건립료일충감서연부병항성감정적방법：매개품충취3괴중등대소적서괴，경엄격소독량간후，절서괴중부서편，매서괴절3개서편，매서편후도약위8 mm，치우방유멸균습윤려지보습적멸균배양명중，접충1괴6 mm적감서연부병균균설우서편중앙，장접충호적서편치우26℃생화배양상중배양，기간가무균수보습1차。21 h후장감정재료취출，량취서편발병직경，근거병반직경진행분급，계산병정지수，진행항감평개。해방법적건립위항병품충적선육제공료이론의거。이용해방법대44빈품충자원진행항성평개，결과현시，로서4호화엄자8호대감서연부병적항성급별위중항；담서271、태중11、투자1호、공서1호화PZJ상27 E2-2대감서연부병적항성급별위감병；기여품충대감서연부병적항성급별균위고감。
According to the incidence of sweet potato infected by Rhizopus nigricans Ehvb.,we established an identification method of sweet potato soft rot resistance for the first time:3 sweet potatos of median size were taken each variety ,after strictly disinfected by 75%alcohol and dried ,3 potato chips were cut in the middle every sam-ple,each chip′s thickness was about 8 mm,then put each chip into a sterilization moist petri dish ,with Rhizopus nigricans Ehvb.inoculated on the center of potato chips ,then put these petri dishes into a biochemical incubator which temperature was 26℃,added sterile water to keep moist .21 hours after the identification of material was cul-tured,the scab diameter was measured ,disease incidence was calculated according to the average scab diameter of grading ,and then identity the anti-sense characteristics of varieties .The establishment of this method provide a theo-retical basis for the breeding of sweet potato .The resistances of 44 varieties of resources to sweet potato soft rot were identified and evaluated ,The results indicated that Lushu 4 and Guangzi 8 showed middle resistance to sweet potato soft rot;Zhanshu 271,Taizhong 11,Yuzi 1,Ganshu 1 and PZJShang 27E2-2 showed sensitive to sweet potato soft rot,all the other sweet potato varieties were high-sensitive to sweetpotato soft rot .