CAJ | 학술논문

为了研究FT／TFL类基因山葡萄雌花和雄花中的表达模式，以山葡萄3个雄花品系和3个雌花品系为材料，根据GenBank上已经发表的3个葡萄FT／TFL类基因设计引物，利用实时荧光定量PCR技术研究VvFT、VvTFL1A和VvTFL1B这3个基因在山葡萄雌花和雄花中的表达。3个基因在山葡萄雌花和雄花中都有表达。 VvFT基因在雌花中的表达量显著高于雄花。而VvTFL1A和VvTFL1B这2个基因在雄花中的表达量略高于雌花。 VvFT基因的表达高峰出现在花序刚开始展露期（5月14日）；而VvTFL1A和VvTFL1B表达高峰出现在芽体膨大期（4月30日）。结果表明，这两类基因在山葡萄花发育过程中作用时间不同。 FT类基因可能参与花的前期调控，而TFL类基因在花器官发育中起作用。
위료연구FT／TFL류기인산포도자화화웅화중적표체모식，이산포도3개웅화품계화3개자화품계위재료，근거GenBank상이경발표적3개포도FT／TFL류기인설계인물，이용실시형광정량PCR기술연구VvFT、VvTFL1A화VvTFL1B저3개기인재산포도자화화웅화중적표체。3개기인재산포도자화화웅화중도유표체。 VvFT기인재자화중적표체량현저고우웅화。이VvTFL1A화VvTFL1B저2개기인재웅화중적표체량략고우자화。 VvFT기인적표체고봉출현재화서강개시전로기（5월14일）；이VvTFL1A화VvTFL1B표체고봉출현재아체팽대기（4월30일）。결과표명，저량류기인재산포도화발육과정중작용시간불동。 FT류기인가능삼여화적전기조공，이TFL류기인재화기관발육중기작용。
To study FT/TFL genes expression pattern in female and male floral of V itis amurensis Rupr.,three male and three female strains were used to study the expression of three FT/TFL genes (VvFT,VvTFL1A and VvTFL1B) by Real-time PCR technology.The three genes expressed not only in female but also in male floral .The relative expression of VvFT gene in female were significantly higher than in male floral ,while the relative expression of VvTFL1A and VvTFL1B genes in male flowers slightly higher than in female floral .The peak value of VvFT gene expression appeared at inflorescence show stage (May 14);while VvTFL1A and VvTFL1B expression appeared one peak value at bud intumescence stage ( April 30 ) .It was suggested that FT genes may be involved in regulation of early flowers,and TFL genes played a role in floral organ development .