CAJ | 학술논문

为了更快更准确地在病菌潜伏期或发病初期鉴定出尖孢镰刀菌引起的瓜类枯萎病和茄病镰刀菌引起的瓜类根腐病这2种病害，根据尖孢镰刀菌和茄病镰刀菌的翻译延长因子TEF1-α序列设计了3条种特异性引物。引物对FO-F和Fu-R可以从尖孢镰刀菌中扩增到1条228 bp的片段，引物对FS-F和Fu-R可以从茄病镰刀菌中扩增到1条347 bp片段，并且3条引物FO-F、FS-F和Fu-R可以同时在1个PCR反应中扩增到2个片段，而这2对引物都不能从其他病原真菌中扩增到任何条带。结果表明，该多重PCR检测方法可以同时鉴定样品中的2种镰刀菌。
위료경쾌경준학지재병균잠복기혹발병초기감정출첨포렴도균인기적과류고위병화가병렴도균인기적과류근부병저2충병해，근거첨포렴도균화가병렴도균적번역연장인자TEF1-α서렬설계료3조충특이성인물。인물대FO-F화Fu-R가이종첨포렴도균중확증도1조228 bp적편단，인물대FS-F화Fu-R가이종가병렴도균중확증도1조347 bp편단，병차3조인물FO-F、FS-F화Fu-R가이동시재1개PCR반응중확증도2개편단，이저2대인물도불능종기타병원진균중확증도임하조대。결과표명，해다중PCR검측방법가이동시감정양품중적2충렴도균。
The early symptoms caused by F.oxysporum and F.solani were similar in cucurbitaceous plants such as watermelon .To rapidly and accurately distinguish the two diseases at a potential or early infection ,three primes were designed based on the TEF 1-α( Translation elongation factor 1-α) of F.oxysporum and F.solani.The primer pair FO-F+Fu-R amplified a 228 bp fragment from F.oxysporum,and the primer pair FS-F+Fu-R amplified a 347 bp fragment from F.solani.No PCR products were amplified with these primer pairs from DNA of other fungal spe-cies.A multiple species-specific PCR method was developed successfully to detect both two fusarium fungi simulta -neously in single PCR amplifications with the primers FO-F+FS-F+Fu-R.