CAJ | 학술논문

为了筛选到具有木质部组织特异性并有较强功能的启动子，将河北林木种质资源与森林保护重点实验室前期已获得的MDCesA启动子、JCesAP启动子、DMDCesAP启动子（含2个串联MDCesAP启动子）融合GUS报告基因的pMDCesAP-GUS、pJCesAP-GUS、pDMDCesAP-GUS这3个植物表达载体，通过农杆菌介导转化杨树，对转化后的杨树进行GUS活性的定性及荧光定量检测，以比较3个启动子在转基因植物中的表达能力和水平。筛选到的组织特异启动子与抗天牛基因融合，将提高毒蛋白在相应部位的表达量，这对天牛类蛀干害虫的防治具有重大意义。
위료사선도구유목질부조직특이성병유교강공능적계동자，장하북림목충질자원여삼림보호중점실험실전기이획득적MDCesA계동자、JCesAP계동자、DMDCesAP계동자（함2개천련MDCesAP계동자）융합GUS보고기인적pMDCesAP-GUS、pJCesAP-GUS、pDMDCesAP-GUS저3개식물표체재체，통과농간균개도전화양수，대전화후적양수진행GUS활성적정성급형광정량검측，이비교3개계동자재전기인식물중적표체능력화수평。사선도적조직특이계동자여항천우기인융합，장제고독단백재상응부위적표체량，저대천우류주간해충적방치구유중대의의。
In order to screen the xylem tissue specific and strong function promoter ,three plant expression vec-tor pMDCesAP-GUS,pJCesAP-GUS,pDMDCesAP-GUS,fused with GUS report gene and MDCesA promoter ,JCesAP promoter ,DMDCesAP promoter ( including two series MDCesAP promoter ) acquired earlier ,are transformed to pop-lar by agrobacterium mediated transformation .With qualitative and quantitative fluorescence detection of GUS activi-ty,the three promoter in transgenic plants expressing ability and level are detected .Fusion of the screened xylem specific promoter and insect-resistant gene will improve the poisonous protein expression in the xylem of the quanti-ty,which is of great significance for prevention and control of boring beetles .