CAJ | 학술논문

为研究小鼠Dazl基因的功能及探究Dazl基因过表达在干细胞向生殖细胞分化过程中的作用，采用RT-PCR方法，从13．5 dpc 雌性胎鼠卵巢中克隆出Dazl基因的全部编码区，测序正确后利用限制性内切酶将其连接到真核表达载体pcDNA3．1上。线性化后对小鼠成纤维细胞进行转染，qRT-PCR及Western Blot 技术显示外源基因Dazl已经成功转染并表达。为研究Dazl基因的功能及干细胞向生殖细胞的诱导分化奠定了基础。
위연구소서Dazl기인적공능급탐구Dazl기인과표체재간세포향생식세포분화과정중적작용，채용RT-PCR방법，종13．5 dpc 자성태서란소중극륭출Dazl기인적전부편마구，측서정학후이용한제성내절매장기련접도진핵표체재체pcDNA3．1상。선성화후대소서성섬유세포진행전염，qRT-PCR급Western Blot 기술현시외원기인Dazl이경성공전염병표체。위연구Dazl기인적공능급간세포향생식세포적유도분화전정료기출。
To study the function of Dazl gene and to confirm its promotion role during the differentiation of stem cells to germ cells,the full coding sequence of Dazl gene was cloned from the ovaries of female mice of 13.5 days post coitum (dpc).Then it was ligated into eukaryotic expression vector pcDNA 3.1 after digested by the restriction endonuclease HindⅢand KpnⅠ.The mouse fibroblasts were transfected using the linearized pcDNA 3.1-Dazl.The results of qRT-PCR and Western Blot showed the foreign gene Dazl had transfected into eukaryotic cells .The study would provide the foundations for the induction and differentiation of stem cells to germ cells .