CAJ | 학술논문

目的：检测慢性牙周炎患者唾液β-葡萄糖醛酸苷酶（β-glucuronidase，β-G）水平，并与治疗后及健康人群对照，以期明确β-G与慢性牙周炎的关系，为牙周炎诊断及疗效监测提供有效参考。方法选择18名慢性牙周炎患者，采集牙周基础治疗前及治疗后全唾液并记录牙周临床指数；检测全唾液中β-G（比色法）水平，并分析与牙周临床参数之间的相关性。结果（1）基础治疗后，牙周临床参数改善明显（P＜0．05）；（2）治疗前实验组唾液β-G活性水平（2．845±0．72，10－3U· mL－1）与健康对照组（2．24±0．31，10－3 U· mL－1）差异有显著性（P＜0．05），与平均牙周袋深度（r ＝0．485）、深袋数（r ＝0．540）、深袋构成比（r＝0．598）之间明显相关（P＜0．05）；治疗后β-G酶活性水平（2．18±0．71，10－3 U· mL－1）明显下调（P＜0．05），与对照组差异无显著性（P＞0．05）。结论唾液β-G活性水平能在一定程度上反映当前牙周炎状态及控制程度，该指标可为牙周炎诊断和疗效监测提供参考。
목적：검측만성아주염환자타액β-포도당철산감매（β-glucuronidase，β-G）수평，병여치료후급건강인군대조，이기명학β-G여만성아주염적관계，위아주염진단급료효감측제공유효삼고。방법선택18명만성아주염환자，채집아주기출치료전급치료후전타액병기록아주림상지수；검측전타액중β-G（비색법）수평，병분석여아주림상삼수지간적상관성。결과（1）기출치료후，아주림상삼수개선명현（P＜0．05）；（2）치료전실험조타액β-G활성수평（2．845±0．72，10－3U· mL－1）여건강대조조（2．24±0．31，10－3 U· mL－1）차이유현저성（P＜0．05），여평균아주대심도（r ＝0．485）、심대수（r ＝0．540）、심대구성비（r＝0．598）지간명현상관（P＜0．05）；치료후β-G매활성수평（2．18±0．71，10－3 U· mL－1）명현하조（P＜0．05），여대조조차이무현저성（P＞0．05）。결론타액β-G활성수평능재일정정도상반영당전아주염상태급공제정도，해지표가위아주염진단화료효감측제공삼고。
Objective The aims of study were to investigate the changes of the salivary levels ofβ-G in patients with chronic periodonti-tis before and after initial periodontal therapy,and evaluate the relationship between the content of the enzyme and periodontal clinical parameters.Methods Eighteen patients with moderate to severe chronic periodontitis were selected.They first underwent supragingival scaling and oral health instruction ( OHI) .A week later,non-stimulated whole saliva was collected and periodontal clinical parameters were examined and the patients were treated with subgingival scaling and root planning.The salivaryβ-G was examined by flourometric assay.A month later,the salivary levels ofβ-G were reevaluated.t test was used to analyze the differences of salivary enzymes levels and periodontal parameters before and after initial periodontal therapy;pearson correlation was used to analyze the correlation between the levels of enzymes and periodontal parameters.Results (1) The changes in the clinical periodontal variables were significant following initial periodontal therapy;(2) At baseline,the salivaryβ-G activity of chronic periodontitis patients was statistically higher than that of controls (P<0.05),and correlated significantly with mean probing depth (MPD) and the number of sites with probing depth≥6 mm. Following initial periodontal therapy,the salivaryβ-G activity decreased significantly.Conclusion The salivaryβ-G activity could be a reflection of current status of chronic periodontitis.The detection of the enzyme may therefore aid clinician in the diagnosis and thera-peutic evaluation of the disease.