北京医学
北京醫學
북경의학
BEIJING MEDICAL JOURNAL
2015年
2期
157-159
,共3页
杨玲玲%石丽萍%龚晓燕%陈立%任芙蓉
楊玲玲%石麗萍%龔曉燕%陳立%任芙蓉
양령령%석려평%공효연%진립%임부용
血小板保存%血小板膜糖蛋白%流式细胞术
血小闆保存%血小闆膜糖蛋白%流式細胞術
혈소판보존%혈소판막당단백%류식세포술
Platelet preservation%Platelet membrane glycoprotein%Flow cytometry(FCM)
目的:测定冷藏(4℃)保存48 h后血小板膜糖蛋白GPⅠbα末端糖基脱落情况。方法分别以一定浓度梯度的FITC-sWGA、FITC-RCA-1、FITC-ECA标记血小板,应用流式细胞仪检测,确定最佳工作浓度。检测冷藏保存48 h后血小板GPⅠbα末端唾液酸基及次末端半乳糖基脱落情况。结果 FITC-sWGA、FITC-RCA-1、FITC-ECA工作浓度分别为2μg/ml、2μg/ml、30μg/ml时标记效果好。冷藏保存48 h后GPⅠbα末端唾液酸基及次末端半乳糖基脱落较22℃常规保存明显增加,分别增至(156±18)%及(123±13)%,差异有统计学意义(P<0.01)。结论冷藏保存48 h可致血小板膜糖蛋白GPⅠbα末端糖基脱落增加。
目的:測定冷藏(4℃)保存48 h後血小闆膜糖蛋白GPⅠbα末耑糖基脫落情況。方法分彆以一定濃度梯度的FITC-sWGA、FITC-RCA-1、FITC-ECA標記血小闆,應用流式細胞儀檢測,確定最佳工作濃度。檢測冷藏保存48 h後血小闆GPⅠbα末耑唾液痠基及次末耑半乳糖基脫落情況。結果 FITC-sWGA、FITC-RCA-1、FITC-ECA工作濃度分彆為2μg/ml、2μg/ml、30μg/ml時標記效果好。冷藏保存48 h後GPⅠbα末耑唾液痠基及次末耑半乳糖基脫落較22℃常規保存明顯增加,分彆增至(156±18)%及(123±13)%,差異有統計學意義(P<0.01)。結論冷藏保存48 h可緻血小闆膜糖蛋白GPⅠbα末耑糖基脫落增加。
목적:측정랭장(4℃)보존48 h후혈소판막당단백GPⅠbα말단당기탈락정황。방법분별이일정농도제도적FITC-sWGA、FITC-RCA-1、FITC-ECA표기혈소판,응용류식세포의검측,학정최가공작농도。검측랭장보존48 h후혈소판GPⅠbα말단타액산기급차말단반유당기탈락정황。결과 FITC-sWGA、FITC-RCA-1、FITC-ECA공작농도분별위2μg/ml、2μg/ml、30μg/ml시표기효과호。랭장보존48 h후GPⅠbα말단타액산기급차말단반유당기탈락교22℃상규보존명현증가,분별증지(156±18)%급(123±13)%,차이유통계학의의(P<0.01)。결론랭장보존48 h가치혈소판막당단백GPⅠbα말단당기탈락증가。
Objective To measure the exposed residues of platelet membrane glycoprotein GPⅠbαwith flow cy-tometry (FCM) after refrigerating 48 h. Methods A series of concentration was set, and the optimal working concentration of FITC-sWGA, FITC-RCA-1, FITC-ECA was determined respectively. The platelet membrane GPⅠbαterminal exposed residues were analyzed after 22℃ preservation and 4℃ refrigeration respectively. Results FITC-sWGA, FITC-RCA-1 and FITC-ECA worked well at 2μg/ml, 2μg/ml and 30μg/ml respectively. Refrigeration led to increasing of GPⅠbαterminal sugar radicals shedding, terminal sialic acid group shedding increased to (156±18)%(P< 0.01), and penultimate galactose group shedding increased to (123 ±13)%(P< 0.01). Conclusion Refrigerating platelets for 48 h leads to in-creasing of platelet GPⅠbαsubunit terminal sugar radicals shedding.