河北医科大学学报
河北醫科大學學報
하북의과대학학보
JOURNAL OF HEBEI MEDICAL UNIVERSITY
2015年
2期
170-172
,共3页
吕翠环%李玉静%孙志平%李卓%吕莎%郑立恒
呂翠環%李玉靜%孫誌平%李卓%呂莎%鄭立恆
려취배%리옥정%손지평%리탁%려사%정립항
结核,脑膜%染色与标记%实时聚合酶链反应
結覈,腦膜%染色與標記%實時聚閤酶鏈反應
결핵,뇌막%염색여표기%실시취합매련반응
tuberculosis,meningeal%staining and labeling%real-time polymerase chain reaction
目的：评价脑脊液改良抗酸染色法与实时荧光定量聚合酶链反应（fluorescence quantitative polymerase chain reaction，FQ-PCR）法诊断结核性脑膜炎（tuberculous meningitis，TBM）的意义。方法 TBM 患者81例，采集脑脊液检测标本，分别采用离心甩片改良抗酸染色法、实时 FQ-PCR 法检测抗酸杆菌。结果81例改良抗酸染色法阳性率66．7％（54／81）高于 FQ-PCR 方法阳性率50．6％（41／81），差异有统计学意义（P ＜0．05）。结论在诊断 TBM 方面，改良抗酸染色方法显著优于 FQ-PCR 方法。
目的：評價腦脊液改良抗痠染色法與實時熒光定量聚閤酶鏈反應（fluorescence quantitative polymerase chain reaction，FQ-PCR）法診斷結覈性腦膜炎（tuberculous meningitis，TBM）的意義。方法 TBM 患者81例，採集腦脊液檢測標本，分彆採用離心甩片改良抗痠染色法、實時 FQ-PCR 法檢測抗痠桿菌。結果81例改良抗痠染色法暘性率66．7％（54／81）高于 FQ-PCR 方法暘性率50．6％（41／81），差異有統計學意義（P ＜0．05）。結論在診斷 TBM 方麵，改良抗痠染色方法顯著優于 FQ-PCR 方法。
목적：평개뇌척액개량항산염색법여실시형광정량취합매련반응（fluorescence quantitative polymerase chain reaction，FQ-PCR）법진단결핵성뇌막염（tuberculous meningitis，TBM）적의의。방법 TBM 환자81례，채집뇌척액검측표본，분별채용리심솔편개량항산염색법、실시 FQ-PCR 법검측항산간균。결과81례개량항산염색법양성솔66．7％（54／81）고우 FQ-PCR 방법양성솔50．6％（41／81），차이유통계학의의（P ＜0．05）。결론재진단 TBM 방면，개량항산염색방법현저우우 FQ-PCR 방법。
Objective To evaluate united test significance in the diagnosis of tuberculous meningitis (TBM)with modified acid-fast staining method of cerebrospinal fluid and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR).Methods Cerebrospinal fluid samples from 81 patients with TBM were detected for Mycobacterium tuberculosis respectively with centrifugal modified acid-fast staining method and real-time FQ-PCR.Results The positive rate of modified acid-fast staining 66.7%(54/81)was significantly higher than that of FQ-PCR 50.6%(41/81),there was significant difference between the two methods(P <0.05).Conclusion Modified acid-fast staining method is significantly better than FQ-PCR in the diagnosis of TBM.
