CAJ | 학술논문

目的：评价结核感染T细胞检测（tuberculous infection of T cells spot test，T-SPOT．TB）在疑似骨关节结核病中的诊断价值。方法选取80例确诊为骨关节结核患者和44例排除结核患者。分别进行抗酸染色、结核菌培养、实时荧光定量 PCR 检测及 T-SPOT．TB，比较并分析检测结果。结果 T-SPOT．TB 的敏感度、特异度和准确度分别为82．5％、75．0％、79．8％。T-SPOT．TB 的阳性检出率显著高于抗酸染色、结核分枝杆菌培养（P ＜0．05），而在不同疾病部位（脊柱、四肢关节）及不同病程分组间阳性检出率差异无统计学意义（P ＞0．05）。结论 T-SPOT．TB 可以作为骨关节结核病的重要辅助诊断方法。
목적：평개결핵감염T세포검측（tuberculous infection of T cells spot test，T-SPOT．TB）재의사골관절결핵병중적진단개치。방법선취80례학진위골관절결핵환자화44례배제결핵환자。분별진행항산염색、결핵균배양、실시형광정량 PCR 검측급 T-SPOT．TB，비교병분석검측결과。결과 T-SPOT．TB 적민감도、특이도화준학도분별위82．5％、75．0％、79．8％。T-SPOT．TB 적양성검출솔현저고우항산염색、결핵분지간균배양（P ＜0．05），이재불동질병부위（척주、사지관절）급불동병정분조간양성검출솔차이무통계학의의（P ＞0．05）。결론 T-SPOT．TB 가이작위골관절결핵병적중요보조진단방법。
Objective To investigate the value of tuberculous infection of T cells spot test (T-SPOT.TB)in the diagnosis of osteoarticular tuberculosis.Methods The study enrolled 80 patients who were confirmed osteoarticular tuberculosis and 44 did not have active tuberculosis. Acid-fast staining,mycobacterial culture,real-time quantitative PCR were respectively performed for the patients.The results were compared and analyzed.Results The sensitivity,specificity, accuracy for osteoarticular tuberculosis diagnosis by T-SPOT.TB assay were 82.5%,75.0% and 79.8%.The positive rate of T-SPOT.TB was significantly higher than that of acid-fast staining, mycobacterial culture(P < 0.05).But,there were no statistical difference in different locations (spine or extremities joint)and courses between groups.Conclusion The results suggested that T-SPOT.TB assay could provide useful aids in the diagnosis of osteoarticular tuberculosis.