CAJ | 학술논문

用多种荧光标记物进行STR检测时，由于荧光光谱的谱带展宽特性，各个荧光光谱之间有重叠部分，如何实现将相互重叠的各个波长上的能量有效利用起来，对提高荧光利用效率至关重要。本文给出了一种基于矩阵分析的数据处理方法，该方法在进行荧光探测前首先要对荧光谱进行光谱校正，即要得到每种所用标记染料受激发射荧光的光谱分布，根据光谱分布建立染料组合荧光信号矩阵，然后对矩阵进行归一，利用归一后的矩阵对所探测的荧光谱进行解谱，从而得到所期望的荧光谱图。理论分析和实验结果表明，该方法可以有效的利用各个波长的荧光光谱能量，并实现对不同荧光重叠谱的有效光谱解谱。
용다충형광표기물진행STR검측시，유우형광광보적보대전관특성，각개형광광보지간유중첩부분，여하실현장상호중첩적각개파장상적능량유효이용기래，대제고형광이용효솔지관중요。본문급출료일충기우구진분석적수거처리방법，해방법재진행형광탐측전수선요대형광보진행광보교정，즉요득도매충소용표기염료수격발사형광적광보분포，근거광보분포건립염료조합형광신호구진，연후대구진진행귀일，이용귀일후적구진대소탐측적형광보진행해보，종이득도소기망적형광보도。이론분석화실험결과표명，해방법가이유효적이용각개파장적형광광보능량，병실현대불동형광중첩보적유효광보해보。
In a detection system of STR labeled by multiple fluorescence,the overlaps do occur between the different colored flu-orophores owing to the band broadening characteristic of the employed fluorescence spectrum.It is crucial to obtain effective en-ergy distribution specific for different wavelengths so as to improve the utilization efficiency of fluorescence intensity.To reach this goal in practice,we propose a data processing method based on matrix analysis.Firstly,a spectral calibration must be done for STR fluorescence spectral analysis,and then a emission spectral distribution of each colored fluorophore excitated by laser is created from the detection signal.And then,the matrix normalization is computed from the above fixed spectrum distribution. And lastly,the inversion of the correct matrix is used to get the resolved and desirable STR spectrogram.In addition,perform-ance evaluation shows that this method based on matrix analysis can make use of the fluorescence spectrum energy of each wave-length and resolve the overlapping spectrum effectively.