CAJ | 학술논문

固定激发波长320 nm，对不同浓度百菌清药液进行荧光光谱实验，发现在352和366 nm处有明显特征峰，随着药液浓度降低，366 nm肩峰逐渐消失，而352 nm特征峰保持稳定；对百菌清浓度和所得发射光谱荧光强度（352 nm）进行指数函数回归分析，相关系数为0．999，实验结果与荧光强度-浓度理论计算公式相符合；对低浓度药液浓度和荧光强度进行线性拟合，其百菌清残留预测模型函数相关系数为0．995，最低检出限为0．0188μg·mL－1，定量极限值为0．0627μg·mL－1，线性范围为0．0627～28．45μg·mL－1。通过对中药材黄芪和枸杞与百菌清混合体系的荧光光谱进行研究，发现两种中药材对百菌清荧光强度都有较强的衰减，表明它们都和百菌清发生了相互作用。经过分析计算，黄芪和枸杞的衰减率分别为88．5％和99．7％，对其建立强度衰减模型函数，相关系数分别为0．994和0．997。研究结果为利用荧光光谱检测百菌清残留提供了实验依据，表明可以采用荧光光谱方法直接对百菌清农药残留进行检测，相关参数值满足检测要求标准，这为进一步利用荧光光谱检测该农药在果蔬中的残留具有重要的参考价值。同时发现药食同源类中药材枸杞和黄芪都能对百菌清荧光特征峰强度产生显著衰减，这为研究利用药食同源类中药材降解农药残留提供了新的研究思路。
고정격발파장320 nm，대불동농도백균청약액진행형광광보실험，발현재352화366 nm처유명현특정봉，수착약액농도강저，366 nm견봉축점소실，이352 nm특정봉보지은정；대백균청농도화소득발사광보형광강도（352 nm）진행지수함수회귀분석，상관계수위0．999，실험결과여형광강도-농도이론계산공식상부합；대저농도약액농도화형광강도진행선성의합，기백균청잔류예측모형함수상관계수위0．995，최저검출한위0．0188μg·mL－1，정량겁한치위0．0627μg·mL－1，선성범위위0．0627～28．45μg·mL－1。통과대중약재황기화구기여백균청혼합체계적형광광보진행연구，발현량충중약재대백균청형광강도도유교강적쇠감，표명타문도화백균청발생료상호작용。경과분석계산，황기화구기적쇠감솔분별위88．5％화99．7％，대기건립강도쇠감모형함수，상관계수분별위0．994화0．997。연구결과위이용형광광보검측백균청잔류제공료실험의거，표명가이채용형광광보방법직접대백균청농약잔류진행검측，상관삼수치만족검측요구표준，저위진일보이용형광광보검측해농약재과소중적잔류구유중요적삼고개치。동시발현약식동원류중약재구기화황기도능대백균청형광특정봉강도산생현저쇠감，저위연구이용약식동원류중약재강해농약잔류제공료신적연구사로。
The fluorescence spectrum was studied for the chlorothalonil(0. 292 8 mg·mL-1 )using spectroflu-orophotometer.The experiment results showed that the characteristic peaks (352 and 366 nm)are found in the spectrum of chlorothalonil standard solution when the excitation wavelength is 320 nm.And it was found that the shoulder peak gradually disappeared at 366 nm,while the fluorescence peak is stable at 352 nm with the decline of the solution concentration.The exponential functional relationship between the concentration of chlorothalonil and fluorescence intensity at 352 nm was obtained,and its correlation coefficient is 0. 999.The experimental results are consistent with the theoretical formula about fluorescence intensity and concentration. The prediction model functions were also obtained through the liner fitting to the chlorothalonil solution of low concentration,and the correlation coefficient is 0. 995.The limit of detection (LOD)is 0. 018 8μg·mL-1 , the limit of quantification (LOQ)is 0. 062 7μg·mL-1 ,and the linear range is 0. 062 7~28. 45μg·mL-1 . And fluorescence spectra were studied for the mixed system of astragalus,medlar and chlorothalonil.It was found that the fluorescence intensity of chlorothalonil solution is all declined with the addition of two kinds of Chinese Herbal Medicines,which indicates that there is an interaction between them.The decay rate of fluo-rescence intensity was obtained which is 88. 5% and 99. 7%,respectively.Then the model functions were es-tablished between fluorescence intensity and the volume of addition,and the correlation coefficient is 0. 994 and 0. 997,respectively.This study provides the experimental foundation for the detection of chlorothalonil resi-dues using fluorescence spectrum.It is shown that it is possible to detect pesticide residues of chlorothalonil using fluorescence spectra directly,and the relevant parameter value satisfied the requirement of testing stand-ard.Therefore there is an important value for further detecting the pesticide residues in fruit juice using fluo-rescence spectrum.It was also found that the fluorescence intensity of chlorothalonil is decreased with the ad-dition of astragalus or medlar,which provides the new research approach to studying the pesticide degradation using medicinal and edible Chinese Herbal Medicines.