CAJ | 학술논문

采用衰减全反射-傅里叶变换红外光谱法（ATR-FTIR）对64份鱼翅干制品（包括真鱼翅、仿鱼翅和素鱼翅）及明胶增重真鱼翅干制品进行快速无损检测鉴别。实验比较了上述四种样品的特征红外光谱图，结果显示，同一种样品的红外光谱具有较好的稳定性；四种鱼翅干制品的红外光谱图存在明显的差异，主要表现在蛋白质酰胺Ⅰ和Ⅱ峰（1650，1544 cm－1）和多糖骨架（1050 cm－1）等营养成分吸收峰的差异。真鱼翅样品由于蛋白质含量高、多糖组分含量较低，故其红外光谱图主要呈现强度较高的蛋白质特征吸收酰胺Ⅰ和Ⅱ峰（1650，1544 cm－1）与较弱的多糖骨架C—O—C伸缩振动峰（1050 cm－1），前者峰面积和（Apro）与后者峰面积和（Apol ）的比值（Apro／Apol ）范围为5．58～25．38。仿鱼翅样品是由鲨鱼的边脚料、胶体及其他一些物质混合、压模而成的，其他添加物质导致减弱了蛋白质特征吸收酰胺Ⅰ和Ⅱ峰的强度，也使酰胺Ⅰ峰发生了约30 cm－1的蓝移，Apro／Apol范围为2．63～2．86。素鱼翅样品主要是由海藻酸钠等多糖类物质合成的，其红外光谱图上几乎不显示蛋白质的特征峰，而多糖的特征峰（～1047 cm－1）表现明显，高于真鱼翅相应吸收峰的强度，Apro／Apol范围为0．60～0．72。真鱼翅样品涂覆明胶增重后，样品表面的红外光谱图显示蛋白质和多糖的特征峰均有所减弱，Apro／Apol值为1．16，而样品剖面的红外光谱图则与真鱼翅的谱图基本一致。可见，采用ATR-FTIR，利用样品间特征红外光谱图和Apro／Apol值的差异，可简便、快速、直观地实现鱼翅干制品品质的无损快速鉴别。
채용쇠감전반사-부리협변환홍외광보법（ATR-FTIR）대64빈어시간제품（포괄진어시、방어시화소어시）급명효증중진어시간제품진행쾌속무손검측감별。실험비교료상술사충양품적특정홍외광보도，결과현시，동일충양품적홍외광보구유교호적은정성；사충어시간제품적홍외광보도존재명현적차이，주요표현재단백질선알Ⅰ화Ⅱ봉（1650，1544 cm－1）화다당골가（1050 cm－1）등영양성분흡수봉적차이。진어시양품유우단백질함량고、다당조분함량교저，고기홍외광보도주요정현강도교고적단백질특정흡수선알Ⅰ화Ⅱ봉（1650，1544 cm－1）여교약적다당골가C—O—C신축진동봉（1050 cm－1），전자봉면적화（Apro）여후자봉면적화（Apol ）적비치（Apro／Apol ）범위위5．58～25．38。방어시양품시유사어적변각료、효체급기타일사물질혼합、압모이성적，기타첨가물질도치감약료단백질특정흡수선알Ⅰ화Ⅱ봉적강도，야사선알Ⅰ봉발생료약30 cm－1적람이，Apro／Apol범위위2．63～2．86。소어시양품주요시유해조산납등다당류물질합성적，기홍외광보도상궤호불현시단백질적특정봉，이다당적특정봉（～1047 cm－1）표현명현，고우진어시상응흡수봉적강도，Apro／Apol범위위0．60～0．72。진어시양품도복명효증중후，양품표면적홍외광보도현시단백질화다당적특정봉균유소감약，Apro／Apol치위1．16，이양품부면적홍외광보도칙여진어시적보도기본일치。가견，채용ATR-FTIR，이용양품간특정홍외광보도화Apro／Apol치적차이，가간편、쾌속、직관지실현어시간제품품질적무손쾌속감별。
Sixty-four pieces of shark fin dried products (including real,fake and artificial shark fin products)and real products coated with gelatin were rapidly and nondestructively analyzed by attenuated total reflection-Fourier transform infrared spectros-copy (ATR-FTIR).The characteristic of IR spectrograms among the above four kinds of samples were systematically studied and comparied,the results showed that the spectrograms of the same kind of samples were repeatable,and different kinds of shark fin products presented significant differences in the spectrograms,which mainly manifested as the specific absorption peaks of amido bonds in protein (1 650,1 544 cm-1 )and skeletal vibration in polysaccharide (1 050 cm-1 ).The spectrograms of real shark fins were characterized by the strong absorption peaks of protein characteristic amide Ⅰ and Ⅱ absorbent (1 650,1 544 cm-1 )and relatively weak C—O—C vibration absorbent (1 050 cm-1 )owing to the high content of protein and relatively low level of polysaccharide.For fake shark fin products that were molded form by mixing together with the offcut of shark,collagen and other substances,the introduction of non-protein materials leaded to the weaker amido bonds absorbent than real products along with a 30 cm-1 blue shift of amide Ⅰabsorbent.Opposite to the real sample,the relatively strong absorption peak of poly-saccharide (~1 047 cm-1 )and barely existed amide absorbent were the key features of the spectrogram of artificial samples, which was synthersized by polysaccharide like sodium alginate.Real samples coated with gelatin,the peak strength of protein and polysaccharide were decreased simultaneously when the data collection was taken at the surface of sample,while the spectro-gram presented no significant difference to real samples when the data was collected in the section.The results above indicated that by analyzing the characteristic of IR spectrograms and the value range of Apro/Apol collected by ATR-FTIR method could perform the undamaged and rapid identification for shark fins.