CAJ | 학술논문

目的：进一步探究流式细胞术（FCM）检测血小板相关抗体（PAIg）在免疫性血小板减少症（ITP）中的应用价值。方法选取我院于2011年12月～2013年12月收治的50例免疫性血小板减少症患者，将其设定为观察组，同时选取同期来我院进行体检的50例健康者作为对照组，两组均采用流式细胞术对血小板相关抗体进行检测，分析患者治疗前后血小板相关抗体的表达率和荧光强度。结果经检测后，观察组患者荧光阳性百分率和平均荧光强度均明显高于对照组，其血小板与巨核细胞数量与对照组差异具有统计学意义（P＜0.05）。结论将流式细胞术应用于免疫性血小板减少症患者中，能够有效检测出血小板相关抗体的变化情况，具有较高的诊断价值，在临床上具有极为重要的意义，值得推广。
목적：진일보탐구류식세포술（FCM）검측혈소판상관항체（PAIg）재면역성혈소판감소증（ITP）중적응용개치。방법선취아원우2011년12월～2013년12월수치적50례면역성혈소판감소증환자，장기설정위관찰조，동시선취동기래아원진행체검적50례건강자작위대조조，량조균채용류식세포술대혈소판상관항체진행검측，분석환자치료전후혈소판상관항체적표체솔화형광강도。결과경검측후，관찰조환자형광양성백분솔화평균형광강도균명현고우대조조，기혈소판여거핵세포수량여대조조차이구유통계학의의（P＜0.05）。결론장류식세포술응용우면역성혈소판감소증환자중，능구유효검측출혈소판상관항체적변화정황，구유교고적진단개치，재림상상구유겁위중요적의의，치득추엄。
Objective To explore the flow cytometry (FCM) to detect platelet-associated antibodies (PAIg) applications in immune thrombocytopenia (ITP) in. Methods Our hospital in December 2011 admitted in December 2013 during the 50 cases of patients with immune thrombocytopenia, which is set in the observation group, and select the same period came to our hospital for medical examination of 50 cases of healthy subjects as controls group, both groups using lfow cytometry to detect platelet antibodies, expression analysis before and after treatment platelet-associated antibodies and fluorescence intensity. Results After testing, the percentage of fluorescence positive patients in the observation group and the mean fluorescence intensity was signiifcantly higher platelet and megakaryocyte number of signiifcant differences with the control group (P<0.05). Conclusion The lfow cytometry applied to patients with autoimmune thrombocytopenia, and can effectively detect changes in platelet-associated antibodies, has a high diagnostic value, has a very important signiifcance in clinical practice, worthy of promotion.