CAJ | 학술논문

目的：研究自主跑轮运动对小鼠骨骼肌线粒体蛋白输入机制（protein import machin‐ery ，PIM ）组件的影响，对 PIM在运动科学方面的研究进行初探，从而为运动促进健康的线粒体方面的机制研究提供新的实验依据。研究方法：选用清洁级4周龄雄性C57BL／6小鼠20只，随机分为安静对照组（N组）和自主跑轮运动组（E组）。 E组小鼠采用8周自主跑轮运动，在运动干预期结束，禁食12 h后断颈处死，并迅速取出小鼠左、右股四头肌，用 RT‐PCR检测线粒体 PIM 组件线粒体外膜转位酶（ Tom 20，Tom 40）、线粒体内膜转位酶（Tim22）、VDAC3、线粒体生物发生关键分子P53，线粒体 PIM伴侣分子 HSPA1和内质网应激分子Bip的转录水平；用 Western‐blot 检测 P53、Tom 40、Tim22和 HSPA1的蛋白表达水平。结果：自主跑轮运动对 Tom 40、Tom 20、VDAC3和Bip分子的转录水平均有显著影响（ P＜0．05），尤其是 Tom40，但对P53和 TIM22的 mRNA 含量没有明显影响（P＞0．05）；自主跑轮运动可明显增加 HSPA1的蛋白表达量（ P＜0．05），但显著降低了 Tim22的蛋白表达量（ P＜0．01），且跑轮运动对 P53和 Tom 40的蛋白表达量无明显影响（ P＞0．05）。结论：自主跑轮运动可增加小鼠骨骼肌线粒体 PIM经典途径组件 Tom 40、Tom 20、VDAC3，以及内质网应激分子Bip的转录水平，同时，自主跑轮运动亦上调了PIM 伴侣分子 HSPA1的蛋白表达，但下调了 PIM非经典途径组件 Tim22的蛋白表达，说明跑轮运动对不同的PIM途径组件可能有不同的影响。
목적：연구자주포륜운동대소서골격기선립체단백수입궤제（protein import machin‐ery ，PIM ）조건적영향，대 PIM재운동과학방면적연구진행초탐，종이위운동촉진건강적선립체방면적궤제연구제공신적실험의거。연구방법：선용청길급4주령웅성C57BL／6소서20지，수궤분위안정대조조（N조）화자주포륜운동조（E조）。 E조소서채용8주자주포륜운동，재운동간예기결속，금식12 h후단경처사，병신속취출소서좌、우고사두기，용 RT‐PCR검측선립체 PIM 조건선립체외막전위매（ Tom 20，Tom 40）、선립체내막전위매（Tim22）、VDAC3、선립체생물발생관건분자P53，선립체 PIM반려분자 HSPA1화내질망응격분자Bip적전록수평；용 Western‐blot 검측 P53、Tom 40、Tim22화 HSPA1적단백표체수평。결과：자주포륜운동대 Tom 40、Tom 20、VDAC3화Bip분자적전록수평균유현저영향（ P＜0．05），우기시 Tom40，단대P53화 TIM22적 mRNA 함량몰유명현영향（P＞0．05）；자주포륜운동가명현증가 HSPA1적단백표체량（ P＜0．05），단현저강저료 Tim22적단백표체량（ P＜0．01），차포륜운동대 P53화 Tom 40적단백표체량무명현영향（ P＞0．05）。결론：자주포륜운동가증가소서골격기선립체 PIM경전도경조건 Tom 40、Tom 20、VDAC3，이급내질망응격분자Bip적전록수평，동시，자주포륜운동역상조료PIM 반려분자 HSPA1적단백표체，단하조료 PIM비경전도경조건 Tim22적단백표체，설명포륜운동대불동적PIM도경조건가능유불동적영향。
Objective :This study was focused on the effect of voluntary running wheel exercise on mitochondrial protein import machinery (PIM ) components in mice skeletal muscle .We ex‐plored the PIM in terms of sports science ,so as to provide new experimental evidence for the mechanism of mitochondria in the movement promoting the health .Methods :We chose 20 clean 4‐week‐old male C57BL/6 mice ,which were randomly divided into control group (N ) and voluntary running wheel exercise group (E) .Group E proceeded 8 weeks running wheel move‐ment .At the end of the exercise intervention ,the mice were sacrificed after fasting 12 hours , removed the quadriceps quickly .We assayed the mRNA level of PIM components ‐ mitochon‐drial translocase of the outer membrane (Tom20 ,Tom40 ) ,mitochondrial translocase of the inner membrane (Tim22) ,voltage dependent anion channel (VDAC3) ,the key molecule of mi‐tochondrial biogenesis P53 ,PIM chaperone HSPA 1 and endoplasmic reticulum stress molecules Bip with RT‐PCR .Simultaneously ,we detected the protein content of P 53、Tom40、Tim22 and HSPA1 with Western‐blot .Results :The effect of voluntary running wheel exercise on the transcriptional level of Tom40 ,Tom20 ,VDAC3 and Bip molecules were significant ( P <0 .05) ,especially on Tom40 ,but there were no significant effect on the mRNA levels of P53 and TIM22 (P> 0 .05) .The voluntary running wheel movement significantly increased the protein expression of HSPA 1 ( P< 0 .05 ) ,but significantly reduced the protein content of Tim22 ( P< 0 .01 ) .However ,there were no significant effect of running wheel exercise on protein expression of P53 and Tom40 ( P> 0 .05 ) .Conclusion :The voluntary running wheel exercise can promote gene expression of classical PIM pathway components (Tom40 ,Tom20 , VDAC3) ,and endoplasmic reticulum stress molecules Bip ,while the movement raises the pro‐tein expression of chaperone HSPA1 of PIM .But voluntary running wheel movement down regulates the protein expression of non‐classical PIM pathway components Tim22 ,indicating that there may be different affection of exercise on different PIM pathways .