CAJ | 학술논문

目的：探讨慢性肾小球肾炎（CGN ）患者外周血Th1／Th2及CD28＋细胞百分率的变化在疾病进程中可能的细胞免疫学机制。方法采用流式细胞术检测50例 CGN 患者及30名正常人（对照组）外周血 Th1／Th2和 CD28＋细胞的百分率，用 SPSS 16．0软件对试验结果进行统计分析。结果与对照组比较，CGN 患者外周血CD3＋CD4＋细胞百分率和 CD4／CD8比值明显降低（P ＜0．05），Th1和 Th2细胞百分率差异无统计学意义（P ＞0．05），但 Th1／Th2比值明显增高（P ＜0．05）；CGN 患者 CD28＋、CD4＋CD28＋细胞百分率明显减少（P 均＜0．01）。结论CGN 患者体内 T 细胞免疫功能紊乱和活化受限，细胞免疫功能低下，Th1和 Th2细胞失衡，通过对 CGN 患者外周血 Th1／Th2及 CD28＋细胞的检测，有助于了解患者的细胞免疫状态，为临床诊断和免疫治疗提供参考依据。
목적：탐토만성신소구신염（CGN ）환자외주혈Th1／Th2급CD28＋세포백분솔적변화재질병진정중가능적세포면역학궤제。방법채용류식세포술검측50례 CGN 환자급30명정상인（대조조）외주혈 Th1／Th2화 CD28＋세포적백분솔，용 SPSS 16．0연건대시험결과진행통계분석。결과여대조조비교，CGN 환자외주혈CD3＋CD4＋세포백분솔화 CD4／CD8비치명현강저（P ＜0．05），Th1화 Th2세포백분솔차이무통계학의의（P ＞0．05），단 Th1／Th2비치명현증고（P ＜0．05）；CGN 환자 CD28＋、CD4＋CD28＋세포백분솔명현감소（P 균＜0．01）。결론CGN 환자체내 T 세포면역공능문란화활화수한，세포면역공능저하，Th1화 Th2세포실형，통과대 CGN 환자외주혈 Th1／Th2급 CD28＋세포적검측，유조우료해환자적세포면역상태，위림상진단화면역치료제공삼고의거。
Objective To investigate the possible mechanism of cellular immunology in the process of chronic glomerulonephritis (CGN)by the detection of Th1 /Th2 ratio and CD28 + cell percentages from peripheral blood in patients with CGN.Methods Th1 /Th2 ratio and CD28 + cell percentages were detected by flow cytometry from 50 patients with CGN and 30 healthy subjects (control group).SPSS 1 6.0 software was used to analyze the results. Results Compared with control group,the percentages of CD3 +CD4 + cells and CD4 /CD8 ratio of patients with CGN decreased significantly (P <0.05).Although there was no statistical significance in the percentages of Th1 and Th2 cells (P >0.05),yet Th1 /Th2 ratio increased significantly (P <0.05).The percentages of CD28 + and CD4 +CD28 +cells decreased significantly (P <0.01 ).Conclusions T cell immune function is disorder,and activation is restricted in patients with CGN.Their cellular immune function is low,and there exists imbalance between Th1 and Th2 cells. The detection of Th1 /Th2 ratio and percentages of CD28 + cells from peripheral blood in patients with CGN is valuable to understand the immune state so as to provide the theoretical basis for immune therapy.