CAJ | 학술논문

目的：比较MicroflexTM基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和Vitek 2 Compact全自动微生物分析系统（简称 Vitek 2 Compact）对肠杆菌科细菌的鉴定能力。方法采用 MicroflexTM MALDI-TOF MS 和 Vitek 2 Compact 同时对545株肠杆菌科质控菌株和临床分离菌株进行鉴定，鉴定结果不一致者采用沙门菌血清凝集试验或细菌16S rDNA 基因测序予以确证。结果MicroflexTM MALDI-TOF MS 对545株细菌的种和属的鉴定率分别为97．1％、2．9％。Vitek 2 Compact 对545株细菌的种、群、属的鉴定率分别为83．3％、13．9％和2．2％，鉴定错误率为0．2％，未鉴定率为0．4％。结论MicroflexTM MALDI-TOF MS 对肠杆菌科细菌的鉴定符合率高于 Vitek 2 Compact，且操作快速、简便，成本低，可用于临床肠杆菌科细菌的常规快速鉴定。
목적：비교MicroflexTM기질보조격광해흡전리비행시간질보（MALDI-TOF MS）화Vitek 2 Compact전자동미생물분석계통（간칭 Vitek 2 Compact）대장간균과세균적감정능력。방법채용 MicroflexTM MALDI-TOF MS 화 Vitek 2 Compact 동시대545주장간균과질공균주화림상분리균주진행감정，감정결과불일치자채용사문균혈청응집시험혹세균16S rDNA 기인측서여이학증。결과MicroflexTM MALDI-TOF MS 대545주세균적충화속적감정솔분별위97．1％、2．9％。Vitek 2 Compact 대545주세균적충、군、속적감정솔분별위83．3％、13．9％화2．2％，감정착오솔위0．2％，미감정솔위0．4％。결론MicroflexTM MALDI-TOF MS 대장간균과세균적감정부합솔고우 Vitek 2 Compact，차조작쾌속、간편，성본저，가용우림상장간균과세균적상규쾌속감정。
Objective To compare the identification abilities of MicroflexTM matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)and Vitek 2 Compact automatic microbial analysis system (Vitek 2 Compact)for Enterobacteriaceae.Methods A total of 545 isolates of Enterobacteriaceae from a variety of clinical and quality control isolates were identified by MicroflexTM MALDI-TOF MS and Vitek 2 Compact.The isolates were confirmed by 1 6S rDNA gene sequencing,and Salmonella was identified by serum agglutination test,if the identification results of the 2 systems didn′t match.Results The coincidence rates of 545 isolates of Enterobacteriaceae identified by MicroflexTM MALDI-TOF MS were 97.1 % to the species level and 2.9% to the genus level,respectively. The coincidence rates of 545 isolates of Enterobacteriaceae identified by Vitek 2 Compact were 83.3%,1 3.9% and 2.2% to the species,group and genus levels,respectively.The false rate was 0.2%,and the un-identification rate was 0.4%.Conclusions The identification coincidence rate of Enterobacteriaceae by MicroflexTM MALDI-TOF MS is higher than that of Vitek 2 Compact.Using MicroflexTM MALDI-TOF MS to identify Enterobacteriaceae is fast,simple to operate and low-cost,and it can be used in routine rapid identification of Enterobacteriaceae in clinical microbiology laboratories.