CAJ | 학술논문

目的：通过研究gp96、髓样细胞白血病-1（Mcl-1）蛋白在肝硬化和肝癌组织中的表达及临床病理学意义，初步探讨其与肝硬化和肝癌发生、发展的关系。方法采用免疫组织化学 ENVISION 法分别检测19例肝硬化组织（癌旁肝硬化组织）、32例肝癌组织和21例对照肝组织（癌旁非硬化肝组织）中 gp96、Mcl-1的表达，并分析其各自表达与肝癌临床病理学特征的关系。取来源于同一患者的肝癌组织、癌旁肝硬化组织或癌旁非肝硬化组织，分别配对检测 gp96、Mcl-1，比较两者的阳性表达率。结果对照组、肝硬化组和肝癌组 gp96阳性表达率逐渐递增，差异有统计学意义（P ＜0．05）；gp96的阳性表达与肿瘤有无包膜、TNM分期有关（P ＜0．05），与患者的性别、年龄、肿瘤大小、血清甲胎蛋白（AFP）值、组织学分级及临床分期无关（P ＞0．05）。肝癌组和肝硬化组Mcl-1阳性表达率明显高于对照组（P ＜0．05），但肝癌组和肝硬化组之间差异无统计学意义（P ＞0．05）；Mcl-1阳性表达与肿瘤有无坏死和 TNM分期有关（P ＜0．05）。肝癌组 gp96阳性表达率明显高于配对癌旁肝硬化组及配对癌旁非肝硬化组（P 均＜0．05）；而肝癌组 Mcl-1阳性表达率明显高于配对癌旁非肝硬化组，与配对癌旁肝硬化组比较差异无统计学意义（P ＞0．05）。结论gp96、Mcl-1过表达可能与肝癌的发生、发展有关。gp96可能参与了肝硬化的发生、发展及向肝癌的恶性转化，有助于判断肝癌患者的预后。
목적：통과연구gp96、수양세포백혈병-1（Mcl-1）단백재간경화화간암조직중적표체급림상병이학의의，초보탐토기여간경화화간암발생、발전적관계。방법채용면역조직화학 ENVISION 법분별검측19례간경화조직（암방간경화조직）、32례간암조직화21례대조간조직（암방비경화간조직）중 gp96、Mcl-1적표체，병분석기각자표체여간암림상병이학특정적관계。취래원우동일환자적간암조직、암방간경화조직혹암방비간경화조직，분별배대검측 gp96、Mcl-1，비교량자적양성표체솔。결과대조조、간경화조화간암조 gp96양성표체솔축점체증，차이유통계학의의（P ＜0．05）；gp96적양성표체여종류유무포막、TNM분기유관（P ＜0．05），여환자적성별、년령、종류대소、혈청갑태단백（AFP）치、조직학분급급림상분기무관（P ＞0．05）。간암조화간경화조Mcl-1양성표체솔명현고우대조조（P ＜0．05），단간암조화간경화조지간차이무통계학의의（P ＞0．05）；Mcl-1양성표체여종류유무배사화 TNM분기유관（P ＜0．05）。간암조 gp96양성표체솔명현고우배대암방간경화조급배대암방비간경화조（P 균＜0．05）；이간암조 Mcl-1양성표체솔명현고우배대암방비간경화조，여배대암방간경화조비교차이무통계학의의（P ＞0．05）。결론gp96、Mcl-1과표체가능여간암적발생、발전유관。gp96가능삼여료간경화적발생、발전급향간암적악성전화，유조우판단간암환자적예후。
Objective To investigate the expressions and clinical pathological significance of gp96 and myeloid cell leukemia-1 (Mcl-1 )in liver cirrhosis and hepatocellular carcinoma tissues to study preliminarily their relationships with the genesis and progression development of liver cirrhosis and hepatocellular carcinoma.Methods The expressions of gp96 and Mcl-1 were detected respectively by ENVISION immunohistochemistry in 1 9 liver cirrhosis tissues (liver cirrhosis tissues beside hepatocellular carcinoma),32 hepatocellular carcinoma tissues and 21 control tissues (non-liver cirrhosis tissues beside hepatocellular carcinoma ). Their relationships of expressions with clinical pathological characteristics of hepatocellular carcinoma were investigated.The expressions of gp96 and Mcl-1 of liver cirrhosis tissues beside hepatocellular carcinoma,hepatocellular carcinoma tissues and non-liver cirrhosis tissues beside hepatocellular carcinoma from one patient were detected,and the positive expression rates were compared.Results The positive expression rate of gp96 increased gradually from control,liver cirrhosis to hepatocellular carcinoma groups,which showed significantly different (P <0.05).The positive expression of gp96 had relationships with tumor envelope and TNMstaging(P <0.05),and there was no relationship with sex,age,tumor size,serum alpha-fetoprotein (AFP), histological grading and clinical staging (P >0.05).Mcl-1 positive expression in hepatocellular carcinoma and liver cirrhosis groups markedly increased compared to control group,respectively(P <0.05),and there was no statistical significance between liver cirrhosis and hepatocellular carcinoma groups (P >0.05).The positive expression of Mcl-1 had relationships with tumor necrosis and TNM staging (P <0.05 ).The positive expression rate of gp96 in hepatocellular carcinoma group was significantly higher than those in liver cirrhosis and control groups (P <0.05).The positive expression rate of Mcl-1 in hepatocellular carcinoma group was higher than that in control group,and had no statistical significance with that in liver cirrhosis group (P >0.05).Conclusions The overexpressions of gp96 and Mcl-1 are related to the genesis and progression of hepatocellular carcinoma.The gp96 may implicate in the formation and development of liver cirrhosis as well as its subsequent malignant transformation,and may be used as a prognostic biomarker for hepatocellular carcinoma.