CAJ | 학술논문

目的：对白血病典型的4种分型血清样本进行检测分析，建立白血病分型的蛋白质指纹图谱诊断模型。方法应用蛋白质指纹图谱技术检测16例急性粒细胞白血病 M2a、18例慢性粒细胞白血病、14例急性淋巴细胞白血病及16例急性粒细胞白血病 M3，采用 Biomarker Wizard 及 Biomarker Patterns 5．0软件进行标记物的比较判别，建立决策树诊断模型。结果由 m／z 3376、8055及9421建立决策树诊断模型，其中16例急性粒细胞白血病 M2a 检出率为75．0％（12／16）、9例慢性粒细胞白血病检出率为100．0％（18／18）、14例急性淋巴细胞白血病检出率71．4％（10／14）、16例急性粒细胞白血病 M3检出率为87．5％（12／14）。结论由3个蛋白标记峰构成的蛋白质指纹图谱诊断模型为白血病4种分型的诊断提供了新的借鉴和参考。
목적：대백혈병전형적4충분형혈청양본진행검측분석，건립백혈병분형적단백질지문도보진단모형。방법응용단백질지문도보기술검측16례급성립세포백혈병 M2a、18례만성립세포백혈병、14례급성림파세포백혈병급16례급성립세포백혈병 M3，채용 Biomarker Wizard 급 Biomarker Patterns 5．0연건진행표기물적비교판별，건립결책수진단모형。결과유 m／z 3376、8055급9421건립결책수진단모형，기중16례급성립세포백혈병 M2a 검출솔위75．0％（12／16）、9례만성립세포백혈병검출솔위100．0％（18／18）、14례급성림파세포백혈병검출솔71．4％（10／14）、16례급성립세포백혈병 M3검출솔위87．5％（12／14）。결론유3개단백표기봉구성적단백질지문도보진단모형위백혈병4충분형적진단제공료신적차감화삼고。
Objective To establish protein fingerprinting model for the diagnosis of leukemia through determining 4 typical types of leukemia.Methods A total of 1 6 acute myeloblastic leukemia M2a samples,1 8 chronic myelocytic leukemia samples,1 4 acute lymphoblastic leukemia samples and 1 6 acute myeloblastic leukemia M3 samples were determined by protein fingerprinting technology.The comparison of biomarkers was performed by Biomarker Wizard and Biomarker Patterns 5.0 to establish a diagnosis tree model.Results The diagnosis tree model combined with 3 biomarkers (m/z:3 376,8 055 and 9 421 )could detect 75% acute myeloblastic leukemia M2a (1 2 /1 6),1 00.0%chronic myelocytic leukemia (1 8 /1 8),71 .4% acute lymphoblastic leukemia (1 0 /1 4)and 87.5% acute myeloblastic leukemia M3 (1 2 /1 4).Conclusions The protein fingerprinting model established by 3 protein features maybe a novel method for diagnosing 4 types of leukemia.