CAJ | 학술논문

FABP4(Fatty acid binding protein 4)参与细胞内脂肪酸的转运和脂肪酸代谢，是当前研究动物脂肪沉积与代谢的热门候选基因。为了研究 FABP4基因在绵羊尾脂沉积与代谢中的作用，文章采用生物信息学方法分析了 FABP4氨基酸序列在各物种中的保守性；利用半定量 RT-PCR 方法检测了该基因在阿勒泰羊主要组织中的表达；采用饥饿法成功建立了模拟阿勒泰羊尾脂沉积与代谢的动物模型，利用qPCR和iTRAQ(isobaric tags for relative and absolute quantitation)技术同时验证FABP4基因mRNA和蛋白在阿勒泰羊非饥饿组与饥饿组尾脂中的表达变化。序列分析结果表明，绵羊FABP4氨基酸序列在物种间高度保守，提示FABP4基因可能由于其重要的生物功能而在进化中表现出其物种的保守性。组织表达谱结果显示，FABP4 mRNA在阿勒泰羊肠脂与尾脂中均高丰度表达，暗示FABP4基因可能在脂肪中行驶着重要的生理生化功能。qPCR与iTRAQ结果显示，FABP4 mRNA与蛋白在两种极端条件下尾脂中的表达差异均不显著(P>0.05)，表明FABP4基因可能不是绵羊尾脂沉积与代谢两种极端差异表型的决定基因。以上研究结果为进一步研究 FABP4基因在绵羊尾脂中的生物功能奠定了基础。
FABP4(Fatty acid binding protein 4)삼여세포내지방산적전운화지방산대사，시당전연구동물지방침적여대사적열문후선기인。위료연구 FABP4기인재면양미지침적여대사중적작용，문장채용생물신식학방법분석료 FABP4안기산서렬재각물충중적보수성；이용반정량 RT-PCR 방법검측료해기인재아륵태양주요조직중적표체；채용기아법성공건립료모의아륵태양미지침적여대사적동물모형，이용qPCR화iTRAQ(isobaric tags for relative and absolute quantitation)기술동시험증FABP4기인mRNA화단백재아륵태양비기아조여기아조미지중적표체변화。서렬분석결과표명，면양FABP4안기산서렬재물충간고도보수，제시FABP4기인가능유우기중요적생물공능이재진화중표현출기물충적보수성。조직표체보결과현시，FABP4 mRNA재아륵태양장지여미지중균고봉도표체，암시FABP4기인가능재지방중행사착중요적생리생화공능。qPCR여iTRAQ결과현시，FABP4 mRNA여단백재량충겁단조건하미지중적표체차이균불현저(P>0.05)，표명FABP4기인가능불시면양미지침적여대사량충겁단차이표형적결정기인。이상연구결과위진일보연구 FABP4기인재면양미지중적생물공능전정료기출。
FABP4 (Fatty acid binding protein 4) is a hot candidate gene in fat deposition and lipid metabolism and participates in the transport and metabolism of intracellular free fatty acids. We aim to study the role of FABP4 in fat deposition and metabolism of the rump fat in Altay sheep. In this study, bioinformatics method was used to analyze the protein sequence homology among 10 species, and RT-PCR was employed to detect FABP4 tissue profiling of Altay sheep. An animal model simulating the rump fat deposition and metabolism of Altay sheep was established by continuous starvation, and qPCR and iTRAQ (isobaric tags for relative and absolute quantitation) were used to detecte FABP4 mRNA and protein expression changes in the control and continuous starvation groups, respectively. Sequence analysis showed that FABP4 protein sequence is highly conserved among species, suggesting an important biological function during evolution for FABP4. The RT-PCR result confirmed that FABP4 mRNA was highly expressed in intestinal and rump fat, suggesting that FABP4 plays an important physiological role in fat tissues. We did not find significant differences in FABP4 mRNA and protein between control and continuous starvation groups (P>0.05), which indicates that FABP4 may not be the key gene in fat deposition and metabolism in Altay sheep.The results above lay a foundation for further studies of FABP4 in rump or tail fat.