CAJ | 학술논문

目的：分析温州汉族人群9个STR基因座（D18S1364、D12S391、D13S325、D6S1043、D2S1172、D11S2368、D22-GATA198B05、D8S1132、D7S3048）的等位基因及基因型频率分布。方法：从无血缘关系的355例温州汉族个体的抗凝血中提取DNA，用STR_Typer_10_v1试剂盒对9个STR基因座进行复合PCR扩增，用AB公司310遗传分析仪和GeneMapper ID 3.2v软件作STR分型，用PowerState V12.xls分析软件进行等位基因频率和法医学常用参数统计分析。结果：该9个基因座检出15、12、9、17、15、13、11、10、12个等位基因，9个基因座基因型频率分布均符合Hardy-Weignberg平衡（P＞0.05）；观测杂合度大于0.7831，多态信息含量均大于0.7666，个体识别能力（Dp）均大于0.9266。结论：温州汉族人群的9个基因座均具有较高的遗传多态性，是较理想的遗传标记系统，本研究所得数据可为温州汉族人群法医个体识别、亲权鉴定及遗传学研究提供依据。
목적：분석온주한족인군9개STR기인좌（D18S1364、D12S391、D13S325、D6S1043、D2S1172、D11S2368、D22-GATA198B05、D8S1132、D7S3048）적등위기인급기인형빈솔분포。방법：종무혈연관계적355례온주한족개체적항응혈중제취DNA，용STR_Typer_10_v1시제합대9개STR기인좌진행복합PCR확증，용AB공사310유전분석의화GeneMapper ID 3.2v연건작STR분형，용PowerState V12.xls분석연건진행등위기인빈솔화법의학상용삼수통계분석。결과：해9개기인좌검출15、12、9、17、15、13、11、10、12개등위기인，9개기인좌기인형빈솔분포균부합Hardy-Weignberg평형（P＞0.05）；관측잡합도대우0.7831，다태신식함량균대우0.7666，개체식별능력（Dp）균대우0.9266。결론：온주한족인군적9개기인좌균구유교고적유전다태성，시교이상적유전표기계통，본연구소득수거가위온주한족인군법의개체식별、친권감정급유전학연구제공의거。
Objective:To analyze the alleles and genotype frequencies of nine short tandem repeats loci, those are D18S1364, D12S391, D13S325, D6S1043, D2S1172, D11S2368, D22-GATA198B05, D8S1132, D7S3048, of Han Ethnic Population in Wenzhou. Methods:extract DNA respectively from anticoagulant of 355 unrelated individual samples of Han Ethnic population living in Wenzhou. Deal the nine STR loci of the samples with multiple PCR ampliifcation by STR_Typer_10_v1 kit. Analyze the PCR products by 310 genetic analyzer and GeneMapper ID 3.2v software of AB company. Deal the results with statistical analysis on the allele frequen-cy and common forensic parameters by PowerState V12.xls software. Results:15, 12, 9, 17, 15, 13, 11, 10 and 12 alleles were observed respectively from the nine STR loci. The distribution of genotype frequencies matched the Hardy-Weinberg equilibrium, P was more than 0.05. The heterozygote was more than 0.7831. The poly-morphism information content was more than 0.7666. The combined power of discrimination was more than 0.9266. Conclusion:All of the nine STR loci of Han Ethnic Population in Wenzhou in this study have high genetic polymorphism, and it can be used as good genetic markers. The data obtained in this study can be used in individual identiifcation, paternity test and population genetics investigation of Han Ethnic population living in Wenzhou.