CAJ | 학술논문

目的：通过离体的高分辨核磁共振氢谱（1H NMR）技术研究2型糖尿病db/db小鼠小脑的代谢变化。方法：采用8只15周龄2型糖尿病模型db/db小鼠为实验组，11只15周龄野生正常小鼠为对照组，对其小脑组织进行离体的1H NMR检测分析。结果：实验组与对照组相比小脑的代谢模式明显不同，甘氨酸（Gly）、天冬氨酸（Asp）、谷氨酸（Glu）、γ-氨基丁酸（GABA）、N-乙酰天门冬氨酸（NAA）、丙氨酸（Ala）等代谢物浓度明显下降，差异有统计学意义（P＜0.05或P＜0.01）；谷氨酰胺（Gln）、牛磺酸（Tau）、乳酸（Lac）等代谢物水平显著上升，差异有统计学意义（P＜0.05或P＜0.01）。结论：代谢组学结果说明2型糖尿病模型db/db小鼠小脑中糖代谢、氨基酸代谢出现了紊乱，这为进一步认识糖尿病脑病的发病机制提供了线索。
목적：통과리체적고분변핵자공진경보（1H NMR）기술연구2형당뇨병db/db소서소뇌적대사변화。방법：채용8지15주령2형당뇨병모형db/db소서위실험조，11지15주령야생정상소서위대조조，대기소뇌조직진행리체적1H NMR검측분석。결과：실험조여대조조상비소뇌적대사모식명현불동，감안산（Gly）、천동안산（Asp）、곡안산（Glu）、γ-안기정산（GABA）、N-을선천문동안산（NAA）、병안산（Ala）등대사물농도명현하강，차이유통계학의의（P＜0.05혹P＜0.01）；곡안선알（Gln）、우광산（Tau）、유산（Lac）등대사물수평현저상승，차이유통계학의의（P＜0.05혹P＜0.01）。결론：대사조학결과설명2형당뇨병모형db/db소서소뇌중당대사、안기산대사출현료문란，저위진일보인식당뇨병뇌병적발병궤제제공료선색。
Objective:To investigate the diabetes-related metabolic changes in the cerebellum of db/db mice. Methods:Specimens of the cerebellum were dissected from the 17-week db/db mice and the age-matched controls. The cerebellum tissue samples were extracted and analyzed by 1H NMR-based metabonomics. Results:Compared to the controls, the db/db mice exhibited markedly different metabolic proifles, which included sig-niifcant decrease in the levels of glycine (P<0.01), aspartate (P<0.01), glutamate (P<0.01), GABA (P<0.01), N-acetyl-aspartate (P<0.01) and alanine (P<0.05) and increase in the levels of glutamine (P<0.01), taurine (P<0.05) and lactate (P<0.01). Conclusion:These metabolic changes suggest that glucose metabolism and amino acid metabolism in the cerebellum of db/db mice are disordered, which provides clues for further understanding the pathogenesis of diabetic encephalopathy.