CAJ | 학술논문

目的：探讨脑5-羟色胺（5-HT）及其旁路关键酶在术后疲劳综合征（POFS）大鼠模型中的变化。方法：将64只SD实验大鼠随机分为模型组和对照组（各分为术后1、3、7、10 d组），模型组行70%中段小肠切除端端吻合术，对照组仅行开关腹手术。分别于术后1、3、7、10 d取脑组织，采用高效液相色谱-串联质谱法测定海马组织中5-HT的含量，荧光定量PCR法检测海马组织中色氨酸羟化酶1、2（TPH1、TPH2）和吲哚胺-2，3-二加氧酶（IDO）的表达量。结果：与对照组相比，模型组大鼠5-HT的含量分别于术后1、3 d明显升高（P＜0.05）；在模型组大鼠中，术后1、3 d 5-HT的含量均较术后7、10 d明显升高（P＜0.05），术后3 d升至最高（P＜0.05），术后7、10 d逐渐恢复正常。与对照组相比，模型组大鼠TPH1的表达量于术后3 d明显升高（P＜0.05）；在模型组大鼠中，术后3 d TPH1的表达量较术后1、7、10 d明显升高（P＜0.05）。与对照组相比，模型组大鼠TPH2的表达量分别于术后1、3 d明显升高（P＜0.05）；在模型组大鼠中，术后1、3 d TPH2的表达量均较术后7、10 d明显升高（P＜0.05），术后3 d升至最高，术后7、10 d逐渐恢复正常。与对照组相比，模型组IDO的表达量于术后1 d明显升高（P＜0.05）；在模型组大鼠中，术后1 d IDO的表达量较术后3、7、10 d明显升高（P＜0.05）。结论：在POFS大鼠模型中，5-HT的含量术后早期明显升高，其升高与5-HT限速酶TPH1、TPH2的表达量上调密切相关。
목적：탐토뇌5-간색알（5-HT）급기방로관건매재술후피로종합정（POFS）대서모형중적변화。방법：장64지SD실험대서수궤분위모형조화대조조（각분위술후1、3、7、10 d조），모형조행70%중단소장절제단단문합술，대조조부행개관복수술。분별우술후1、3、7、10 d취뇌조직，채용고효액상색보-천련질보법측정해마조직중5-HT적함량，형광정량PCR법검측해마조직중색안산간화매1、2（TPH1、TPH2）화신타알-2，3-이가양매（IDO）적표체량。결과：여대조조상비，모형조대서5-HT적함량분별우술후1、3 d명현승고（P＜0.05）；재모형조대서중，술후1、3 d 5-HT적함량균교술후7、10 d명현승고（P＜0.05），술후3 d승지최고（P＜0.05），술후7、10 d축점회복정상。여대조조상비，모형조대서TPH1적표체량우술후3 d명현승고（P＜0.05）；재모형조대서중，술후3 d TPH1적표체량교술후1、7、10 d명현승고（P＜0.05）。여대조조상비，모형조대서TPH2적표체량분별우술후1、3 d명현승고（P＜0.05）；재모형조대서중，술후1、3 d TPH2적표체량균교술후7、10 d명현승고（P＜0.05），술후3 d승지최고，술후7、10 d축점회복정상。여대조조상비，모형조IDO적표체량우술후1 d명현승고（P＜0.05）；재모형조대서중，술후1 d IDO적표체량교술후3、7、10 d명현승고（P＜0.05）。결론：재POFS대서모형중，5-HT적함량술후조기명현승고，기승고여5-HT한속매TPH1、TPH2적표체량상조밀절상관。
Objective:To explore the role of changes of key enzymes of brain 5-HT and 5-HT bypass in rat model of postoperative fatigue syndrome. Methods:64 SD rats were randomly divided into model group and con-trol group according to the weigh (which both including 1 d, 3 d, 7 d, 10 d group). The rats of model group would be resected the middle 70%small bowel, and control group only undergoing on-off abdominal surgery. The brains were removed on 1 d, 3 d, 7 d, 10 d postoperatively respectively, and we detected the concentration of 5-HT by high performance liquid chromatography and the mRNA expression of TPH1, TPH2, IDO by realtime lfuores-cence quantitative PCR in hippocampus area. Results:Compared with control group, concentration of 5-HT of model group rats increased signiifcantly on 1 d, 3 d postoperatively respectively (P<0.05). And in model group rats, concentration of 5-HT on 1 d, 3 d increased signiifcantly respectively compared with 7 d, 10 d postoperative-ly (P<0.05), on 3 d to its highest (P<0.05), 7 d, 10 d gradually returned to normal. Compared with control group, mRNA expression of TPH1 in model group rats increased signiifcantly on 3 d postoperatively (P<0.05). And in model group rats, mRNA expression of TPH1 on 3 d increased signiifcantly compared with 1 d, 7 d, 10 d post-operatively (P<0.05). Compared with control group, mRNA expression of TPH2 in model group rats increased signiifcantly on 1 d, 3 d postoperatively respectively (P<0.05). And in model group rats, mRNA expression of TPH2 on 1 d, 3 d increased signiifcantly respectively compared with 7 d, 10 d postoperatively (P<0.05), on 3 d to its highest, 7 d, 10 d gradually returned to normal. Compared with control group, mRNA expression of IDO in model group rats increased signiifcantly on 1d postoperatively (P<0.05). And in model group rats, mRNA expres-sion of IDO on 1 d increased signiifcantly compared with 3 d, 7 d, 10 d postoperatively (P<0.05). Conclusion:In rat model of postoperative fatigue syndrome, concentration of 5-HT increases signiifcantly early postopera-tively. Its mechanism refers to up-regulation of mRNA expression of TPH1, TPH2 of 5-HT rate-limiting enzyme.