癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2015年
1期
26-29,34
,共5页
彭裕辉%谢长乐%庄珊珊%张利群%许镒洧
彭裕輝%謝長樂%莊珊珊%張利群%許鎰洧
팽유휘%사장악%장산산%장리군%허일유
p53%自身抗体%EB病毒%VCA-IgA%鼻咽癌%诊断
p53%自身抗體%EB病毒%VCA-IgA%鼻嚥癌%診斷
p53%자신항체%EB병독%VCA-IgA%비인암%진단
p53%autoantibody%Epstein-Barr virus%VCA-IgA%nasopharyngeal carcinoma%diagnosis
目的：探讨联合检测p53自身抗体与EB病毒在鼻咽癌患者中的诊断意义。方法：采用酶联免疫吸附实验VCA-IgA (ELISA)检测242例鼻咽癌患者和218例正常对照者血清中p53自身抗体和EB病毒壳抗体(VCA-IgA)的表达水平。结果：血清p53自身抗体的水平在鼻咽癌患者组明显高于正常对照组，差异有统计学意义(P<0.01)。p53自身抗体和VCA-IgA对鼻咽癌的诊断敏感度分别为40.1%(95%CI：33.9%~46.6%)和47.5%(95%CI：41.1%~54.0%)；特异度分别为95.0%(95%CI：90.9%~97.3%)和95.4%(95%CI：91.5%~97.7%)，两者联合检测的敏感度和特异度分别为69.4%(95%CI：63.1%~75.0%)和90.8%(95%CI：86.0%~94.2%)。p53自身抗体、VCA-IgA和两者联合检测在早期鼻咽癌患者中的阳性表达均明显高于正常对照组(P均<0.01)。p53自身抗体的阳性表达率与鼻咽癌患者的性别、年龄、T分期、N分期和总TNM分期之间均无明显相关性( P>0.05)。结论：血清p53自身抗体作为一种潜在的诊断标志物，与VCA-IgA联合检测可能有助于筛查和诊断鼻咽癌。
目的：探討聯閤檢測p53自身抗體與EB病毒在鼻嚥癌患者中的診斷意義。方法：採用酶聯免疫吸附實驗VCA-IgA (ELISA)檢測242例鼻嚥癌患者和218例正常對照者血清中p53自身抗體和EB病毒殼抗體(VCA-IgA)的錶達水平。結果：血清p53自身抗體的水平在鼻嚥癌患者組明顯高于正常對照組，差異有統計學意義(P<0.01)。p53自身抗體和VCA-IgA對鼻嚥癌的診斷敏感度分彆為40.1%(95%CI：33.9%~46.6%)和47.5%(95%CI：41.1%~54.0%)；特異度分彆為95.0%(95%CI：90.9%~97.3%)和95.4%(95%CI：91.5%~97.7%)，兩者聯閤檢測的敏感度和特異度分彆為69.4%(95%CI：63.1%~75.0%)和90.8%(95%CI：86.0%~94.2%)。p53自身抗體、VCA-IgA和兩者聯閤檢測在早期鼻嚥癌患者中的暘性錶達均明顯高于正常對照組(P均<0.01)。p53自身抗體的暘性錶達率與鼻嚥癌患者的性彆、年齡、T分期、N分期和總TNM分期之間均無明顯相關性( P>0.05)。結論：血清p53自身抗體作為一種潛在的診斷標誌物，與VCA-IgA聯閤檢測可能有助于篩查和診斷鼻嚥癌。
목적：탐토연합검측p53자신항체여EB병독재비인암환자중적진단의의。방법：채용매련면역흡부실험VCA-IgA (ELISA)검측242례비인암환자화218례정상대조자혈청중p53자신항체화EB병독각항체(VCA-IgA)적표체수평。결과：혈청p53자신항체적수평재비인암환자조명현고우정상대조조，차이유통계학의의(P<0.01)。p53자신항체화VCA-IgA대비인암적진단민감도분별위40.1%(95%CI：33.9%~46.6%)화47.5%(95%CI：41.1%~54.0%)；특이도분별위95.0%(95%CI：90.9%~97.3%)화95.4%(95%CI：91.5%~97.7%)，량자연합검측적민감도화특이도분별위69.4%(95%CI：63.1%~75.0%)화90.8%(95%CI：86.0%~94.2%)。p53자신항체、VCA-IgA화량자연합검측재조기비인암환자중적양성표체균명현고우정상대조조(P균<0.01)。p53자신항체적양성표체솔여비인암환자적성별、년령、T분기、N분기화총TNM분기지간균무명현상관성( P>0.05)。결론：혈청p53자신항체작위일충잠재적진단표지물，여VCA-IgA연합검측가능유조우사사화진단비인암。
OBJECTIVE:To evaluate the diagnostic value of serum autoantibodies against p53 in patients with nasopharyngeal carcinoma. METHODS:The study included 242 patients with NPC and 218 normal controls. Serum levels of autoantibodies against p53 and classical Epstein-Barr virus VCA-IgA were measured by Enzyme-linked immunosorbent assay (ELISA).RESULTS:Serum levels of autoantibodies against p53 were significantly higher in NPC than in normal controls (P<0.01). Measurement of autoantibodies against p53 and VCA-IgA demonstrated a sensitivity/specificity of 40.1% (95%CI:33.9%-46.6%)/95.0% (95%CI:90.9%-97.3%) and 47.5% (95%CI:41.1%-54.0%)/95.4% (95%CI:91.5%-97.7%),respectively. The combination of autoantibodies against p53 and VCA-IgA yielded an enhanced sensitivity of 69.4% (95%CI:63.1%-75.0%) and a similar specificity of 90.8% (95%CI:86.0%-94.2%). Moreover,detection of autoantibodies against p53 could differentiate early stage NPC patients from normal controls. The positive rate of autoantibodies against p53 was not significantly related to age,gender,T stage,N stage or overall stage (P>0.05).CONCLUSION:Autoantibodies against p53 might be used as a potential biomarker supplementary to VCA-IgA for screening and diagnosis of NPC.
