CAJ | 학술논문

SSRs(Simple sequence repeats)是一类广泛存在于动植物基因组的DNA短串联重复序列，是重要的基因组分子标记。比较不同基因组同源SSR的差异，有利于了解相近物种间的进化过程。文章使用雷蒙德氏棉基因组(D5)、亚洲棉基因组(A2)全基因组序列和陆地棉(AD1)的限制性酶切基因组测序数据，进行全基因组SSR扫描，比较了A组和D组的SSR分布情况，通过识别3个基因组之间的同源SSR，比较它们之间同源SSR重复序列的差异。结果发现，A组和D组同源SSR的分布规律非常相似，但A组与AD组的同源SSR保守性比D组与AD组同源SSR的保守性强。与AD组同源SSR相比，A组中重复序列长度增长的SSR数量约为长度缩短的SSR数量的5倍，在D组中这一比值约为3倍。可以推测，四倍体AD组在与A组、D组的平行进化过程中，由于基因组融合，导致SSR的重复序列长度变化速率与二倍体A、D组有差异，同时这种差异可能导致了 AD组 SSR重复序列长度在进化过程中与二倍体相比有变短的趋势。文章首次对3个棉花基因组的同源SSR进行了系统地比较，发现了同源SSR在棉属四倍体基因组和二倍体基因组中的显著差异，为进一步揭示棉属基因组的进化规律提供了基础。
SSRs(Simple sequence repeats)시일류엄범존재우동식물기인조적DNA단천련중복서렬，시중요적기인조분자표기。비교불동기인조동원SSR적차이，유리우료해상근물충간적진화과정。문장사용뢰몽덕씨면기인조(D5)、아주면기인조(A2)전기인조서렬화륙지면(AD1)적한제성매절기인조측서수거，진행전기인조SSR소묘，비교료A조화D조적SSR분포정황，통과식별3개기인조지간적동원SSR，비교타문지간동원SSR중복서렬적차이。결과발현，A조화D조동원SSR적분포규률비상상사，단A조여AD조적동원SSR보수성비D조여AD조동원SSR적보수성강。여AD조동원SSR상비，A조중중복서렬장도증장적SSR수량약위장도축단적SSR수량적5배，재D조중저일비치약위3배。가이추측，사배체AD조재여A조、D조적평행진화과정중，유우기인조융합，도치SSR적중복서렬장도변화속솔여이배체A、D조유차이，동시저충차이가능도치료 AD조 SSR중복서렬장도재진화과정중여이배체상비유변단적추세。문장수차대3개면화기인조적동원SSR진행료계통지비교，발현료동원SSR재면속사배체기인조화이배체기인조중적현저차이，위진일보게시면속기인조적진화규률제공료기출。
Simple sequence repeats (SSRs)are a class of repetitive DNA sequences, which are commonly used for genome analysis. Comparison of the homologous SSRs among different genomes is helpful to understand the evolu-tionary process in relative species. In this study, SSR scanning was performed to investigate their distribution and length variation among the genomes of G. raimondii (D5), G. arboretum (A2) and G. hirsutum (AD1). The results demonstrated that the distribution of SSRs in A genome was very similar with that in D genome, while the length variation of homologous SSRs between A and AD genome was more conserved than that between D and AD genome. Compared with SSRs in AD genome, the number of SSRs with longer motif length in A genome was about five times of those with shorter motif length, while it was about three times in D genome. This implied that the length variation rates of homologous SSRs between diploid cotton and tetraploid cotton were different during the parallel evolution due to the subgenome fusion, and the motif length of most SSRs in tetraoploid genome tended to become shorter than homologous SSRs in diploid genome during the process of evolution. This study comprehensively compared the SSRs in three cotton genomes and revealed the significant difference among them, providing a foundation for further evo-lutionary study of Gossypium genome.