CAJ | 학술논문

目的：探讨不同剂量铁补充对大鼠淋巴细胞DNA损伤的影响。方法：SPF级6~7周龄雄性Wistar大鼠40只，按体质量随机分为4组，每组10只大鼠，即对照组、缺铁组、10倍铁剂量补充组、20倍铁剂量补充组，4组均采用隔日腹腔注射右旋糖酐铁，每2+次注射0.72 mL，每次注射含Fe分别为0.9、0.3、9和18 mg，连续注射6周。4组大鼠自由饮用去离子水，喂饲无铁饲料，于第6周末眼眶取血，使用血清铁试剂盒测定血清铁浓度，采用碱性单细胞凝胶电泳法测定大鼠外周血淋巴细胞DNA损伤状况。结果：缺铁组大鼠血清铁含量为53.54μmol/L，明显低于正常对照组的77.62μmol/L(P<0.01)，10倍和20倍铁剂量补充组大鼠血清平均铁含量分别达到104.77μmol/L和205.30μmol/L，显著高于对照组(P<0.01)。外周血淋巴细胞DNA本底损伤分析显示，缺铁组和正常对照组淋巴细胞DNA损伤总体水平分别为25.30 AU和21.13 AU，两组间差异无统计学意义(P>0.05)，10倍和20倍铁剂量补充组DNA自发损伤水平分别为对照组的3.9倍和8.0倍(82.80 AU和169.50 AU)，显著高于对照组(P<0.01)；然而H2O2与铁过量联合损伤分析显示，大鼠外周血淋巴细胞在10μmol/L H2O2处理后，缺铁组大鼠DNA氧化损伤水平达到260.40 AU，与对照组(259.00 AU)相比差异无统计学意义(P>0.05)，而10倍和20倍铁剂量补充组分别为对照组的1.1倍和1.2倍(293.80 AU和308.88 AU)，均明显高于正常对照组(P<0.01)。结论：10倍、20倍铁剂量补充均可提高机体铁的营养状况或增加铁负荷水平；与正常铁摄入水平相比，铁缺乏未见DNA本底及H2O2联合损伤增加，而铁补充过量可引发机体外周血淋巴细胞DNA本底损伤增加及H2O2联合损伤加剧。
목적：탐토불동제량철보충대대서림파세포DNA손상적영향。방법：SPF급6~7주령웅성Wistar대서40지，안체질량수궤분위4조，매조10지대서，즉대조조、결철조、10배철제량보충조、20배철제량보충조，4조균채용격일복강주사우선당항철，매2+차주사0.72 mL，매차주사함Fe분별위0.9、0.3、9화18 mg，련속주사6주。4조대서자유음용거리자수，위사무철사료，우제6주말안광취혈，사용혈청철시제합측정혈청철농도，채용감성단세포응효전영법측정대서외주혈림파세포DNA손상상황。결과：결철조대서혈청철함량위53.54μmol/L，명현저우정상대조조적77.62μmol/L(P<0.01)，10배화20배철제량보충조대서혈청평균철함량분별체도104.77μmol/L화205.30μmol/L，현저고우대조조(P<0.01)。외주혈림파세포DNA본저손상분석현시，결철조화정상대조조림파세포DNA손상총체수평분별위25.30 AU화21.13 AU，량조간차이무통계학의의(P>0.05)，10배화20배철제량보충조DNA자발손상수평분별위대조조적3.9배화8.0배(82.80 AU화169.50 AU)，현저고우대조조(P<0.01)；연이H2O2여철과량연합손상분석현시，대서외주혈림파세포재10μmol/L H2O2처리후，결철조대서DNA양화손상수평체도260.40 AU，여대조조(259.00 AU)상비차이무통계학의의(P>0.05)，이10배화20배철제량보충조분별위대조조적1.1배화1.2배(293.80 AU화308.88 AU)，균명현고우정상대조조(P<0.01)。결론：10배、20배철제량보충균가제고궤체철적영양상황혹증가철부하수평；여정상철섭입수평상비，철결핍미견DNA본저급H2O2연합손상증가，이철보충과량가인발궤체외주혈림파세포DNA본저손상증가급H2O2연합손상가극。
OBJECTIVE:To investigate the effects of different doses of iron supplementationon lymphocyte DNA damagein rats.METHODS:Forty male Wistar rats were randomly divided into control group,iron deficiency 2+group,10 times-iron as control group and 20 times-iron as control group, containing Fe 0.9,0.3,9 and 18 mg,respectively. All rats were treated with intraperitoneal injection of 0.72 mL iron dextran every other day and the entire study lasted for 6 weeks. The rats in the four groups were provided with deionized water and food without iron,both freely available. After 6 weeks,the level of serum iron was determined by spectrophotomety and the peripheral blood lymphocyte DNA damage was assessed using comet assay.RESULTS:The level of serum iron in the iron deficiency group (53.54μmol/L) was significantly lower than that of control group (77.62μmol/L)(P<0.01). In addition,the levels of serum iron in 10 times-iron (104.77μmol/L) and 20 times-iron groups (205.30μmol/L) were significantly higher than that of control group(P<0.01). DNA damageassessmentindicated that there was no difference between iron deficiency group (25.30 AU) and control group (21.13 AU)(P>0.05). Howeverthe DNA damage of 10 times-iron group (82.80 AU) and 20 times-iron group (169.50 AU) were significantly higher than control group (P<0.01) ,being 3.9 times and 8.0 times, respectively,as control group. The results of combined DNA damage induced by 10μmol/L H2O2 showed that there was no difference between iron deficiency(260.40 AU) group and control group (259.00 AU) (P>0.05). The combined DNA damage of 10 times-iron group (293.80 AU) and 20 times-iron group (308.88 AU) were higher significantly than control group(P<0.01),which were 1.1 times and 1.2 times,respectively,as control group.CONCLUSION:10 times and 20 times iron supplements could improve the iron nutritional status in body or increase the level of iron load. Iron deficiency could not increase the level ofusual DNA damage or combined DNA damage,but iron overload could increase the level of lymphocyte DNA damage andc ombined DNA damage.