CAJ | 학술논문

目的：每组各15只。正常对照组以空气为对照。染毒组给予光气动态染毒5 min，构建光气致急性肺损伤模型。染毒后2 h麻醉，采集肺组织样本，测定肺组织湿/干比，进行肺组织病理检测和电镜观察，并测定肺组织线粒体酶复合物活性。结果：与对照组比较，光气染毒后，肺湿/干比显著增加(P<0.01)，肺组织存在炎细胞浸润和肺泡上皮细胞损伤；线粒体明显肿胀，形态改变，甚至固缩溶解，数量减少，线粒体嵴断裂和缺失，线粒体酶复合体活性降低(P<0.05)。结论：肺泡上皮细胞线粒体损伤为光气中毒的主要毒作用靶点之一，本研究结果为光气中毒防治提供了新思路。研究光气中毒对肺泡上皮细胞线粒体结构和功能的损伤作用。方法：30只SD大鼠随机分为正常对照组和染毒组，3(10 g/m)
목적：매조각15지。정상대조조이공기위대조。염독조급여광기동태염독5 min，구건광기치급성폐손상모형。염독후2 h마취，채집폐조직양본，측정폐조직습/간비，진행폐조직병리검측화전경관찰，병측정폐조직선립체매복합물활성。결과：여대조조비교，광기염독후，폐습/간비현저증가(P<0.01)，폐조직존재염세포침윤화폐포상피세포손상；선립체명현종창，형태개변，심지고축용해，수량감소，선립체척단렬화결실，선립체매복합체활성강저(P<0.05)。결론：폐포상피세포선립체손상위광기중독적주요독작용파점지일，본연구결과위광기중독방치제공료신사로。연구광기중독대폐포상피세포선립체결구화공능적손상작용。방법：30지SD대서수궤분위정상대조조화염독조，3(10 g/m)
OBJECTIVE:To study the toxicological effects of phosgene on pulmonary mitochondria. METHODS:30 SD rats were randomly divided into two groups,with 15 in each group. The rats in control group were 3exposed to air. The rats in phosgene group were exposed to 10 g/m phosgene for 5 min.Two hours after exposure,pulmonary tissues were collected,HE stainingand transmission electron microscope were used to identify histological changes. Activities of mitochondrial complexes were determined. RESULTS:Phosgene induced a significant increase of pulmonary wet/dry ratio. After phosgene poisoning,there were profuse neutrophil infiltration and pulmonary alveoli epithelial cell damage. Mitochondriawerenotably swollen,and even appeared contracted and dissolved. The number of mitochondriawassignificantly decreased. Mitochondrial ridge was broken and absent. The activities of mitochondrial complex were significantly reduced.CONCLUSION:Mitochondriawere main targets of phosgene poisoning,and the results provided basis for the development of new drugs in the treatment of phosgene poisoning.