医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2015年
1期
64-65,69
,共3页
周丽群%苏茶英%胡玉琼%王秉慧
週麗群%囌茶英%鬍玉瓊%王秉慧
주려군%소다영%호옥경%왕병혜
微RNAs%前体细胞淋巴母细胞白血病淋巴瘤%儿童%预后
微RNAs%前體細胞淋巴母細胞白血病淋巴瘤%兒童%預後
미RNAs%전체세포림파모세포백혈병림파류%인동%예후
MicroRNAs%Precursor Cell Lymphoblastic Leukemia-Lymphoma%Child%Prognosis
目的探讨miR‐26a在儿童急性B淋巴细胞白血病(B‐ALL)中的表达及意义。方法收集B‐ALL患儿38例(观察组),以非血液病患儿18例为对照组,采集患儿骨髓标本,提取总RNA ,Realtime PCR检测miR‐26a的表达,比较不同组别、时间和年龄等患儿中miR‐26a的表达。结果观察组中miR‐26a表达显著低于对照组( P <0.05);观察组治疗前miR‐26a表达明显低于治疗d30及第12周( P<0.05),而治疗d30的表达低于第12周( P <0.05);10岁以上患儿组miR‐26a的表达低于10岁以下组( P<0.05);低危组高于中、高危组( P <0.05),而中危组高于高危组( P<0.05)。结论 miR‐26a在儿童B‐ALL中可能存在抑癌作用,可能是预后预测的潜在标志物。
目的探討miR‐26a在兒童急性B淋巴細胞白血病(B‐ALL)中的錶達及意義。方法收集B‐ALL患兒38例(觀察組),以非血液病患兒18例為對照組,採集患兒骨髓標本,提取總RNA ,Realtime PCR檢測miR‐26a的錶達,比較不同組彆、時間和年齡等患兒中miR‐26a的錶達。結果觀察組中miR‐26a錶達顯著低于對照組( P <0.05);觀察組治療前miR‐26a錶達明顯低于治療d30及第12週( P<0.05),而治療d30的錶達低于第12週( P <0.05);10歲以上患兒組miR‐26a的錶達低于10歲以下組( P<0.05);低危組高于中、高危組( P <0.05),而中危組高于高危組( P<0.05)。結論 miR‐26a在兒童B‐ALL中可能存在抑癌作用,可能是預後預測的潛在標誌物。
목적탐토miR‐26a재인동급성B림파세포백혈병(B‐ALL)중적표체급의의。방법수집B‐ALL환인38례(관찰조),이비혈액병환인18례위대조조,채집환인골수표본,제취총RNA ,Realtime PCR검측miR‐26a적표체,비교불동조별、시간화년령등환인중miR‐26a적표체。결과관찰조중miR‐26a표체현저저우대조조( P <0.05);관찰조치료전miR‐26a표체명현저우치료d30급제12주( P<0.05),이치료d30적표체저우제12주( P <0.05);10세이상환인조miR‐26a적표체저우10세이하조( P<0.05);저위조고우중、고위조( P <0.05),이중위조고우고위조( P<0.05)。결론 miR‐26a재인동B‐ALL중가능존재억암작용,가능시예후예측적잠재표지물。
[Objective]To explore the expression and clinical significance of miR‐26a in childhood B‐cell a‐cute lymphoblastic leukemia (ALL) .[Methods]Bone marrow samples were collected from 38 children with B‐cell ALL and 18 controls with non‐hematologic diseases .Total RNA was harvested from bone marrow .Real‐time polymerase chain reaction (PCR) was performed to detect the expression level of miR‐26a .[Results]The relative expression level of miR‐26a was remarkably lower in B‐cell ALL group than that in controls ( P <0 .05) .And the expression of miR‐26a in newly diagnosed samples was lower than those in Day 30 and Week 12 samples respectively ( P <0 .05) .Also its expression in Day 30 samples was lower than those in Week 12 samples ( P<0 .05) .And its expression in children aged over 10 years old was significantly lower than those in children aged under 10 years ( P <0 .05) .Its expression was higher in low‐risk group than those in middle and high‐risk groups respectively ( P <0 .05) .There was significant difference between middle and high‐risk groups ( P <0 .05) .[Conclusion]miR‐26a has tumor suppressor effect so that it may become a potential prog‐nostic biomarker for childhood B cell ALL .