医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2015年
1期
42-44
,共3页
郭琼%严彬%南小新%易路%杨金瑞%傅发军
郭瓊%嚴彬%南小新%易路%楊金瑞%傅髮軍
곽경%엄빈%남소신%역로%양금서%부발군
微RNAs%顺铂%前列腺肿瘤/药物疗法%前列腺肿瘤/病理学%细胞增殖
微RNAs%順鉑%前列腺腫瘤/藥物療法%前列腺腫瘤/病理學%細胞增殖
미RNAs%순박%전렬선종류/약물요법%전렬선종류/병이학%세포증식
MicroRNAs%Cisplatin%Prostatic Neoplasms/DT%Prostatic Neoplasms/PA%Cell Proliferation
目的探讨miRNA‐29b(miR‐29b)在前列腺癌中的表达及意义。方法采用荧光实时定量PCR技术(QRT‐PCR)检测前列腺癌、癌旁非肿瘤组织中miR‐29b的表达,采用M T T法检测miR‐29b的上调对细胞增殖及顺铂化疗作用的影响,用流式细胞术检测细胞周期变化,AV/PI双标法FCM检测细胞的凋亡情况, T ransw ell细胞侵袭实验观察细胞侵袭能力。结果 miR‐29b在前列腺癌组织中的表达明显低于癌旁组织。与阴性对照组相比,miR‐29b模拟物组细胞存活率下降,并且增加顺铂对前列腺癌细胞的抑制作用;miR‐29b模拟物转染组亚G0/G1期明显增加,总凋亡率明显下降,侵袭细胞数量明显减少,差异均有统计学意义( P <0.05)。结论 miR‐29b在前列腺癌中表达下降,增加其表达可以抑制前列腺癌细胞的增殖和侵袭能力,促进癌细胞凋亡并且增加癌细胞对顺铂化疗作用的敏感性。
目的探討miRNA‐29b(miR‐29b)在前列腺癌中的錶達及意義。方法採用熒光實時定量PCR技術(QRT‐PCR)檢測前列腺癌、癌徬非腫瘤組織中miR‐29b的錶達,採用M T T法檢測miR‐29b的上調對細胞增殖及順鉑化療作用的影響,用流式細胞術檢測細胞週期變化,AV/PI雙標法FCM檢測細胞的凋亡情況, T ransw ell細胞侵襲實驗觀察細胞侵襲能力。結果 miR‐29b在前列腺癌組織中的錶達明顯低于癌徬組織。與陰性對照組相比,miR‐29b模擬物組細胞存活率下降,併且增加順鉑對前列腺癌細胞的抑製作用;miR‐29b模擬物轉染組亞G0/G1期明顯增加,總凋亡率明顯下降,侵襲細胞數量明顯減少,差異均有統計學意義( P <0.05)。結論 miR‐29b在前列腺癌中錶達下降,增加其錶達可以抑製前列腺癌細胞的增殖和侵襲能力,促進癌細胞凋亡併且增加癌細胞對順鉑化療作用的敏感性。
목적탐토miRNA‐29b(miR‐29b)재전렬선암중적표체급의의。방법채용형광실시정량PCR기술(QRT‐PCR)검측전렬선암、암방비종류조직중miR‐29b적표체,채용M T T법검측miR‐29b적상조대세포증식급순박화료작용적영향,용류식세포술검측세포주기변화,AV/PI쌍표법FCM검측세포적조망정황, T ransw ell세포침습실험관찰세포침습능력。결과 miR‐29b재전렬선암조직중적표체명현저우암방조직。여음성대조조상비,miR‐29b모의물조세포존활솔하강,병차증가순박대전렬선암세포적억제작용;miR‐29b모의물전염조아G0/G1기명현증가,총조망솔명현하강,침습세포수량명현감소,차이균유통계학의의( P <0.05)。결론 miR‐29b재전렬선암중표체하강,증가기표체가이억제전렬선암세포적증식화침습능력,촉진암세포조망병차증가암세포대순박화료작용적민감성。
[Objective]To explore the expression of miRNA‐29b (miR‐29b) ,examine its effects on cell bi‐ological properties in prostate cancer (Pca) and discuss its potential clinical values .[Methods]The expressions of miR‐29b in prostate cancer tissues and matched adjacent non‐tumor tissues were detected by real‐time quan‐titative polymerase chain reaction (QRT‐PCR) .The cellular growth and chemotherapeutic effect of cisplatin were measured by methyl thiazol tetrazolium (MTT) assay .Flow cytometry was used to determine the cell cy‐cle changes .And AV/PI double labeling method was utilized to detect the apoptosis of LNCap .Finally the im‐pact of miR‐29b on the invasiveness of LNCap cell was determined by Transwell invasion assay .[Results]The expression of miR‐29b was down‐regulated in Pca tissues versus matched adjacent non‐tumor tissues .Com‐pared with negative controls ,group of transfected miR‐29b showed lower tumor cell survival rate .Moreover , miR‐29b could increase the inhibition of cisplatin on LNCap with much lower cell survival rate .LNCap with transfected miR‐29b revealed a higher percentage of cells during sub‐G0/G1 phase and significant decreases of apoptosis cell rate and invasive cell rate ( P <0 .05) .[Conclusion]The expression of miR‐29b is down‐regula‐ted in prostate cancer .A higher expression of miR‐29b inhibits cell proliferation and invasion and induces cell apoptosis in prostate cancer .And the chemosensitivity of cisplatin increases in Pca cell .