CAJ | 학술논문

目的：探讨促红细胞生成素肝细胞（Eph）B4、Eph 受体相互作用蛋白（Ephrin）B2和缺氧诱导因子-1α（HIF-1α）在脑星形细胞瘤组织中的表达水平及意义。方法采用原位杂交法检测120例脑星形细胞瘤组织标本（病例组）和20例脑白质组织标本（对照组）的 EphB4、EphrinB2和 HIF-1α表达水平。结果病例组 EphB4、EphrinB2和 HIF-1α表达水平均明显高于对照组（P ＜0．05），病例组病理分级Ⅲ—Ⅳ级、临床表现有出血及坏死的 EphB4、EphrinB2和 HIF-1α阳性表达率均显著增加（P ＜0．05）。EphB4、EphrinB2表达水平与 HIF-1α表达水平均呈正相关（r＝0．483、0．486，均 P ＜0．05）。结论EphB4、EphrinB2和 HIF-1α在脑星形细胞瘤中的异常表达与肿瘤的恶性程度有关，EphB4与 EphrinB2可能存在协同作用。
목적：탐토촉홍세포생성소간세포（Eph）B4、Eph 수체상호작용단백（Ephrin）B2화결양유도인자-1α（HIF-1α）재뇌성형세포류조직중적표체수평급의의。방법채용원위잡교법검측120례뇌성형세포류조직표본（병례조）화20례뇌백질조직표본（대조조）적 EphB4、EphrinB2화 HIF-1α표체수평。결과병례조 EphB4、EphrinB2화 HIF-1α표체수평균명현고우대조조（P ＜0．05），병례조병리분급Ⅲ—Ⅳ급、림상표현유출혈급배사적 EphB4、EphrinB2화 HIF-1α양성표체솔균현저증가（P ＜0．05）。EphB4、EphrinB2표체수평여 HIF-1α표체수평균정정상관（r＝0．483、0．486，균 P ＜0．05）。결론EphB4、EphrinB2화 HIF-1α재뇌성형세포류중적이상표체여종류적악성정도유관，EphB4여 EphrinB2가능존재협동작용。
Objective To explore the expression and significance of EphB4,EphrinB2 and HIF-1αin human brain astrocytomas.Methods In situ hybridization was performed to detect the ex-pression of EphB4,EphrinB2 and HIF-1αin 120 brain astrocytoma specimens(case group)and 20 cerebral white matter specimens (control group).Results Compared with control group,the ex-pression of EphB4,EphrinB2 and HIF-1αsignificantly increased in case group(P <0.05).Patho-logic stage Ⅲ-Ⅳ grade,clinical manifestations of hemorrhage and necrosis in cose group,the posi-tive expression rate of EphB4,EphrinB2 and HIF-1α significantly increased(P <0.05).The ex-pression of EphB4 and EphrinB2 was positively correlated with the expression of HIF-1α(r =0.483 and 0.486,respectively;P < 0.05).Conclusion The overexpression of EphB4,EphrinB2 and HIF-1αin brain astrocytomas is associated with the malignant degree of tumors.EphB4 and EphrinB2 may be synergies.