CAJ | 학술논문

目的：以健康妇女阴道分泌物为分离源分离乳杆菌，寻求阴道优势乳杆菌，为治疗细菌性阴道疾病提供菌株基础。方法采用传统方法分离鉴定乳杆菌，筛选能产生过氧化氢的优势菌株，并用16SrRNA序列分析方法进行分子鉴定。并验证了其抑菌试验效果。结果从健康妇女阴道中分离到9种乳酸杆菌种属，主要有卷曲乳杆菌，格式乳杆菌，詹氏乳杆菌等，其他也有罗伊氏乳杆菌，约氏乳杆菌，嗜酸乳杆菌和发酵乳杆菌。大多数乳杆菌都能产过氧化氢，且对病原微生物有一定的抑制作用。结论卷曲乳杆菌，格式乳杆菌，詹氏乳杆菌等是妇女阴道内主要的乳杆菌种类，能产过氧化氢，并可以抑制病原菌生长。
목적：이건강부녀음도분비물위분리원분리유간균，심구음도우세유간균，위치료세균성음도질병제공균주기출。방법채용전통방법분리감정유간균，사선능산생과양화경적우세균주，병용16SrRNA서렬분석방법진행분자감정。병험증료기억균시험효과。결과종건강부녀음도중분리도9충유산간균충속，주요유권곡유간균，격식유간균，첨씨유간균등，기타야유라이씨유간균，약씨유간균，기산유간균화발효유간균。대다수유간균도능산과양화경，차대병원미생물유일정적억제작용。결론권곡유간균，격식유간균，첨씨유간균등시부녀음도내주요적유간균충류，능산과양화경，병가이억제병원균생장。
Pbjective To isolate lactobacillus from healthy female Vaginal Secretions,seek the advantage lactobacillus and provide a basis for treating bacterial strains vaginal disease. Methods Using traditional morphology analysis to identify lactobacillus. Screening for hydrogen peroxide—producing lactobacillus. Applying 16SrRNA sequence analysis molecular bi-ology methods to identify it exactly. And had confirmed its bacteriostasis experiment effect. ResuIts L. gasseri,L. crispatus, L. jensenii were the major species isolated from the healthy women,and the other types included L. johnsonii,L. acidophilus and L. fermentum. Most of these lactobacilli can produce hydrogen peroxide,and also had certain inhibitory action to the pathogenic microorganism. ConcIusion L. crispatus,L. jensenii and L. gasseri were predominant species in the vagina of healthy Chinese women and can produce hydrogen peroxide. They had certain inhibitory action to the pathogenic microorgan-ism.