CAJ | 학술논문

目的：对板蓝根的乙酸乙酯部位各化学组分进行体外抗单纯疱疹病毒Ⅰ型（ HSV1）活性研究。方法利用溶剂法和色谱法从板蓝根中提取分离出20个乙酸乙酯组分，利用四甲基偶氮唑盐比色（ MTT）法检测这20个组分体外对HSV1病毒的抑制作用，以细胞存活率、治疗指数（ TI）和细胞保护率为评价指标，比较各化学组分的体外抗病毒的效果。结果板蓝根乙酸乙酯8个组分均有明显的抗 HSV1病毒作用，对病毒的抑制率均远大于50%。结论板蓝根乙酸乙酯部位中存在直接抗病毒较强的活性成分，对其结构进行深入研究，将有助于阐明板蓝根抗病毒药效物质基础。
목적：대판람근적을산을지부위각화학조분진행체외항단순포진병독Ⅰ형（ HSV1）활성연구。방법이용용제법화색보법종판람근중제취분리출20개을산을지조분，이용사갑기우담서염비색（ MTT）법검측저20개조분체외대HSV1병독적억제작용，이세포존활솔、치료지수（ TI）화세포보호솔위평개지표，비교각화학조분적체외항병독적효과。결과판람근을산을지8개조분균유명현적항 HSV1병독작용，대병독적억제솔균원대우50%。결론판람근을산을지부위중존재직접항병독교강적활성성분，대기결구진행심입연구，장유조우천명판람근항병독약효물질기출。
Pbjective To research the antiviral activity against herpes simplex virus typeⅠ(HSV1)activity of each chemical component of Radix Isatidis extracted with ethyl acetate in vitro. Methods Extracting and separating 20 compo-nents from Radix Isatidis in ethyl acetate using solvent and chromatography methods. Detecting this 20 components inhibition to HSV1 virus in vitro using MTT assay,with cell viability,therapeutic index( TI)and cell protection rate as evaluation in-dexes,and comparing the antiviral activities of each component in vitro. ResuIts Eight components of Radix Isatidis had significant anti—viral effect of HSV—1. Inhibition rate to the virus was much greater than 50%. ConcIusion There were strong antiviral of active ingredients in Radix Isatidis. In—depth study of its structure would help to clarify the material basis of antiviral effect of Radix Isatidis.