中国医药生物技术
中國醫藥生物技術
중국의약생물기술
CHINESE MEDICINAL BIOTECHNOLOGY
2015年
1期
45-52
,共8页
纤维蛋白原%生物相容性材料%腹壁缺损%降解%再生%左旋乳酸-聚己内酯
纖維蛋白原%生物相容性材料%腹壁缺損%降解%再生%左鏇乳痠-聚己內酯
섬유단백원%생물상용성재료%복벽결손%강해%재생%좌선유산-취기내지
Fibrinogen%Biocompatible materials%Abdominal wall defect%Degradation%Regeneration%LLA-CL
目的：探讨左旋乳酸-聚己内酯共聚物和纤维蛋白原共混静电纺盆底修复材料应用于盆底脏器脱垂治疗中的体内降解和生物再生特性的研究。方法在30只 SD大鼠左右两侧腹壁建立腹壁缺损区，利用静电纺盆底修复材料对缺损进行修补，一组为补片平铺修补，另一组补片褶皱后修补。术后1、4、8、12、16周各处死6只大鼠，取出修补区域及其周围组织，通过测定聚合物分子量、抗拉强度及电镜观察分析补片在体内的降解、组织的病理变化及再生情况。结果降解16周后，两组机械强度差异无统计学意义，但具有可比性；褶皱组相对分子量大于平铺组（P <0.05）；电镜扫描结果显示两组有明显差异，平铺组补片降解速度快，但无法维持一定的力学强度，褶皱组降解速度慢，但其可保持一定的力学强度；HE 染色、电镜扫描结果都证实两组具有差异性。结论通过两种实验方法对比后发现，平铺组降解虽快，但却不能维持一定的力学强度，因此褶皱植入法优于平铺植入法。
目的：探討左鏇乳痠-聚己內酯共聚物和纖維蛋白原共混靜電紡盆底脩複材料應用于盆底髒器脫垂治療中的體內降解和生物再生特性的研究。方法在30隻 SD大鼠左右兩側腹壁建立腹壁缺損區，利用靜電紡盆底脩複材料對缺損進行脩補，一組為補片平鋪脩補，另一組補片褶皺後脩補。術後1、4、8、12、16週各處死6隻大鼠，取齣脩補區域及其週圍組織，通過測定聚閤物分子量、抗拉彊度及電鏡觀察分析補片在體內的降解、組織的病理變化及再生情況。結果降解16週後，兩組機械彊度差異無統計學意義，但具有可比性；褶皺組相對分子量大于平鋪組（P <0.05）；電鏡掃描結果顯示兩組有明顯差異，平鋪組補片降解速度快，但無法維持一定的力學彊度，褶皺組降解速度慢，但其可保持一定的力學彊度；HE 染色、電鏡掃描結果都證實兩組具有差異性。結論通過兩種實驗方法對比後髮現，平鋪組降解雖快，但卻不能維持一定的力學彊度，因此褶皺植入法優于平鋪植入法。
목적：탐토좌선유산-취기내지공취물화섬유단백원공혼정전방분저수복재료응용우분저장기탈수치료중적체내강해화생물재생특성적연구。방법재30지 SD대서좌우량측복벽건립복벽결손구，이용정전방분저수복재료대결손진행수보，일조위보편평포수보，령일조보편습추후수보。술후1、4、8、12、16주각처사6지대서，취출수보구역급기주위조직，통과측정취합물분자량、항랍강도급전경관찰분석보편재체내적강해、조직적병리변화급재생정황。결과강해16주후，량조궤계강도차이무통계학의의，단구유가비성；습추조상대분자량대우평포조（P <0.05）；전경소묘결과현시량조유명현차이，평포조보편강해속도쾌，단무법유지일정적역학강도，습추조강해속도만，단기가보지일정적역학강도；HE 염색、전경소묘결과도증실량조구유차이성。결론통과량충실험방법대비후발현，평포조강해수쾌，단각불능유지일정적역학강도，인차습추식입법우우평포식입법。
Objective To study the characteristics of intracorporeal degradation and regeneration of electro-spun pelvic floor repair materials composed of LLA-CL and fibrinogen. Methods The abdominal wall defects were created in the left and right sides of the abdominal spaces of 30 SD rats. Then the defects were repaired by electrostatic spinning pelvic floor repair materials. The smooth group was paved followed by repair, and the other group was ruffled before repair. Six rats were sacrificed in each zone of 1, 4, 8, 12, 16 weeks after the operation. The next step was to take out the repaired parts and the surrounding tissues and analyzed the degradation of mesh, the pathologic changes and regeneration of the organization by calculating the molecular weight and the mechanical strength and electron microscopy observation. Results After sixteen weeks degradation, there was no statistically significant difference between the mechanical strength of the two groups, but the results were comparable. The relative molecular weight of the ruffle group surpassed the smooth group (P < 0.05). At the same time, HE staining and electron microscope scanning results have proved that differences existed between the two groups. The speed of patch degradation is faster in the smooth group, but it is unable to maintain a certain mechanical strength. The slow degradation of the ruffle group can keep a certain mechanical strength. Conclusion By comparison with two kinds of experimental methods, we find that the ruffled implantation is better than that of the smooth implants.
