CAJ | 학술논문

为获取γ-PGA高产突变株，确定聚谷氨酸对钙溶解性和对小鼠体内钙吸收的影响。使用紫外照射纳豆芽孢杆菌诱导突变，根据形态特征变化筛选、培养突变株。分离纯化不同突变株的γ-聚谷氨酸（γ-PGA）并测定产量。筛选出γ-PGA高产的突变株并测定其遗传稳定性。测定γ-PGA在体外和体内对钙溶解性的影响以及γ-PGA对小鼠骨矿物质含量的影响。筛选出了具有稳定遗传性的γ-PGA高产突变株Z-29，其γ-PGA的产量大于18 g／L。结果表明，在体外，γ-PGA能促进钙的溶解性；在体内，γ-PGA也能促进小鼠小肠钙的吸收，并能增加小鼠骨钙和骨磷的含量。筛选出了纳豆芽孢杆菌γ-PGA高产的突变株，证明了γ-PGA在体内外都能增加钙的溶解性，这为更深入地研究γ-PGA的功能和生产应用提供了依据。
위획취γ-PGA고산돌변주，학정취곡안산대개용해성화대소서체내개흡수적영향。사용자외조사납두아포간균유도돌변，근거형태특정변화사선、배양돌변주。분리순화불동돌변주적γ-취곡안산（γ-PGA）병측정산량。사선출γ-PGA고산적돌변주병측정기유전은정성。측정γ-PGA재체외화체내대개용해성적영향이급γ-PGA대소서골광물질함량적영향。사선출료구유은정유전성적γ-PGA고산돌변주Z-29，기γ-PGA적산량대우18 g／L。결과표명，재체외，γ-PGA능촉진개적용해성；재체내，γ-PGA야능촉진소서소장개적흡수，병능증가소서골개화골린적함량。사선출료납두아포간균γ-PGA고산적돌변주，증명료γ-PGA재체내외도능증가개적용해성，저위경심입지연구γ-PGA적공능화생산응용제공료의거。
The purpose of this study is to obtain mutant strain with high yields of γ-PGA and determine polyglutamic acid effect on calcium absorption in mice .Bacillus natto was irradiated by ultraviolet to induce muta-tion.The mutant strains were selected according to morphological changes .Then,the mutant strains were cultivated . The γ-poly glutamic acid (γ-PGA) of different mutant strains were separated and purified to determine its produc-tion.The mutant strain which had high yields of γ-PGA was selected and determined its genetic stability .The effects of the calcium solubility by γ-PGA in vitro and in vivo were measured as well as the bone mineral content in mice . Experiment screened out a mutant strain ,Z-29,with stable high yields of γ-PGA greater than 18 g/L.The experi-mental results showed that γ-PGA promoted calcium solubility in vitro and in mice small intestine .Moreover,it in-creased bone calcium and bone phosphorus content in mice .This experiment gained Bacillus natto mutant strains with high yields of γ-PGA and proved that γ-PGA can increase the solubility of calcium in vitro and in vivo.It pro-vided the basis for further research and application in production .