华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
z1期
197-201
,共5页
张徐俞%王瑾瑜%郑广顺%张俊琦%卢存福
張徐俞%王瑾瑜%鄭廣順%張俊琦%盧存福
장서유%왕근유%정엄순%장준기%로존복
树龄%端粒%端粒酶%叶位
樹齡%耑粒%耑粒酶%葉位
수령%단립%단립매%협위
Tree age%Telomere%Telomerase%Leaf position
为探究端粒酶活性与树龄以及叶位是否存在一定的关系,应用TRAP法对不同年龄的油松、银杏、楸树、国槐叶片及油松和银杏同一棵树不同叶位的叶片进行端粒酶活性的测定。结果表明,油松端粒酶活性同树龄无明确的相关性,而楸树、国槐的端粒酶活性随着树龄增加而表现为增加的趋势。端粒酶活性与叶位相关,越靠近顶端的叶片端粒酶活性较高,而靠近下端的叶片端粒酶活性较低。
為探究耑粒酶活性與樹齡以及葉位是否存在一定的關繫,應用TRAP法對不同年齡的油鬆、銀杏、楸樹、國槐葉片及油鬆和銀杏同一棵樹不同葉位的葉片進行耑粒酶活性的測定。結果錶明,油鬆耑粒酶活性同樹齡無明確的相關性,而楸樹、國槐的耑粒酶活性隨著樹齡增加而錶現為增加的趨勢。耑粒酶活性與葉位相關,越靠近頂耑的葉片耑粒酶活性較高,而靠近下耑的葉片耑粒酶活性較低。
위탐구단립매활성여수령이급협위시부존재일정적관계,응용TRAP법대불동년령적유송、은행、추수、국괴협편급유송화은행동일과수불동협위적협편진행단립매활성적측정。결과표명,유송단립매활성동수령무명학적상관성,이추수、국괴적단립매활성수착수령증가이표현위증가적추세。단립매활성여협위상관,월고근정단적협편단립매활성교고,이고근하단적협편단립매활성교저。
The aim of this study was to determine the variation of telomerase during the life-span and with sam-pling position of leaves in different tree species .Therefore ,TRAP assay was used to determine telomerase activity in Pinus tabulaeformis,Ginkgo biloba,Catalpa bungei C.A.Mey,and Sophora japonica Linn.The results showed that no significant difference of telomerase activity was found among different ages of trees .But the higher telomerase ac-tivitives were found in the top position leaves of Pinus tabulaeformis and Ginkgo biloba .The effect of leaf position on the telomerase activity in Pinus tabulaeformis and Ginkgo biloba was strong and significant .This is the first obser-vation on such a position-related variation in telomerase activity .