CAJ | 학술논문

以油橄榄（品种为弗奥）一年生幼苗为试验材料，喷施66．7 mg／L的多效唑（PP333）于油橄榄幼苗叶片表面，每株喷施量为100 mL，且设置对照（喷施相同量的蒸馏水），随后停止浇水，分别取干旱胁迫0，2，7 d的油橄榄幼苗叶片进行各项生理生化指标的测定及比较分析，以期探讨干旱胁迫下叶面喷施多效唑对油橄榄幼苗叶片细胞膜保护系统的影响。结果表明，当干旱胁迫（停止浇水7 d）使细胞膜受到显著破坏时，叶片喷施PP333显著减轻了细胞膜损伤程度，其细胞膜稳定指数比对照（叶片喷施对应量的蒸馏水）高41．5％；研究结果还显示，干旱胁迫下叶面喷施PP333相对减小了油橄榄叶片叶绿素含量、类胡萝卜素含量下降幅度，显著提高了脯氨酸和可溶性糖含量，进一步提高了SOD、POD和CAT活性，抑制MDA含量、O2·－产生速率和H2 O2含量升高的幅度，提高了Vc和还原型谷胱甘肽这2种重要抗氧化物质的含量。可见，干旱胁迫下叶面喷施PP333能通过相对提高干旱胁迫下叶片的抗氧化酶活性、抗氧化物质及渗透调节物质含量，减轻了光合色素含量的下降和活性氧、自由基的累积，由此加强了对油橄榄幼苗叶片细胞膜的保护。
이유감람（품충위불오）일년생유묘위시험재료，분시66．7 mg／L적다효서（PP333）우유감람유묘협편표면，매주분시량위100 mL，차설치대조（분시상동량적증류수），수후정지요수，분별취간한협박0，2，7 d적유감람유묘협편진행각항생리생화지표적측정급비교분석，이기탐토간한협박하협면분시다효서대유감람유묘협편세포막보호계통적영향。결과표명，당간한협박（정지요수7 d）사세포막수도현저파배시，협편분시PP333현저감경료세포막손상정도，기세포막은정지수비대조（협편분시대응량적증류수）고41．5％；연구결과환현시，간한협박하협면분시PP333상대감소료유감람협편협록소함량、류호라복소함량하강폭도，현저제고료포안산화가용성당함량，진일보제고료SOD、POD화CAT활성，억제MDA함량、O2·－산생속솔화H2 O2함량승고적폭도，제고료Vc화환원형곡광감태저2충중요항양화물질적함량。가견，간한협박하협면분시PP333능통과상대제고간한협박하협편적항양화매활성、항양화물질급삼투조절물질함량，감경료광합색소함량적하강화활성양、자유기적루적，유차가강료대유감람유묘협편세포막적보호。
Based on olive(variety Frantoio) annual seedlings as test materials,spraying 66.7 mg/L(PP333) on the surface of olive seedling leaves ,each spraying quantity to 100 mL,and setting a contrast respectively ( spraying the same amount of distilled water),then stop water,drought stress were taken 0,2,and 7 days of olive leaves for the determination of the physiological and biochemical indexes and comparative analysis ,in order to explore the in-fluences of PP333 on olive seedling leaves′cell membrane protective systems under drought stress were studied in this paper.The results showed that cell membrane was damaged significantly under drought stress (stop watering 7 d), but spraying PP333 on the olive seedling leaves significantly reduced the damage degree of the cell membrane ,the data showed that its cell membrane′s stability index was 41 .5%higher than the contrast ( the same amount of dis-tilled water sprayed on the leaves ) after stop watering 7 d.The results also showed that spraying PP 333 on olive seed-ling leaves relatively reduced the reducing range of chlorophyll content and carotenoid content ,increased the content of proline and soluble sugar significantly ,further improved the activity of SOD ,POD and CAT,inhibited the increas-ing range of MDA content ,O2 ·-producing rate and H 2 O2 content ,and even improved the content of the two kinds of important antioxidants ( Vc and reduced glutathione ) under the drought stress significantly .So it can be concluded that through improving the activity of antioxidant enzymes ,enhancing the content of antioxidants and osmotic regula-tion substances ,reducing the decline of photosynthetic pigment content and the accumulation of active oxygen and free radicals,spraying PP333 on the olive seedling leaves promoted the protection on the cell membranes of olive seedling leaves under the drought stress .