CAJ | 학술논문

为了确定在较长期NaCl胁迫条件下，青稞和大麦2种抗逆性较强的禾本科作物的耐盐性表型差异，并为应用比较基因组学技术进一步开发利用禾本科作物抗逆遗传资源打下基础。以1．0％及0．5％NaCl溶液（ w／V）处理的大麦和青稞种子苗为材料，检测分析了这2种植物在NaCl胁迫1～5 d（4 d）条件下的营养生长状况及其对外界胁迫条件的生理响应表型。结果表明，相同NaCl胁迫条件下，青稞种子的萌发率显著高于大麦，而且在较长期的NaCl胁迫条件下青稞种子苗根和叶的生长状况均好于同期大麦。种子苗耐盐生理指标测定结果显示，在相同NaCl胁迫条件下青稞组织内的丙二醛（ Malonyldialdehyde ，MDA）含量极显著低于大麦，而脯氨酸、可溶性糖含量以及超氧化物歧化酶（ Superoxide dismutase ，SOD）比活力则高于大麦，上述结果显示：青稞幼苗比大麦有更好的耐盐性。
위료학정재교장기NaCl협박조건하，청과화대맥2충항역성교강적화본과작물적내염성표형차이，병위응용비교기인조학기술진일보개발이용화본과작물항역유전자원타하기출。이1．0％급0．5％NaCl용액（ w／V）처리적대맥화청과충자묘위재료，검측분석료저2충식물재NaCl협박1～5 d（4 d）조건하적영양생장상황급기대외계협박조건적생리향응표형。결과표명，상동NaCl협박조건하，청과충자적맹발솔현저고우대맥，이차재교장기적NaCl협박조건하청과충자묘근화협적생장상황균호우동기대맥。충자묘내염생리지표측정결과현시，재상동NaCl협박조건하청과조직내적병이철（ Malonyldialdehyde ，MDA）함량겁현저저우대맥，이포안산、가용성당함량이급초양화물기화매（ Superoxide dismutase ，SOD）비활력칙고우대맥，상술결과현시：청과유묘비대맥유경호적내염성。
To analysis the different phenotypes of hulless barley and barley under NaCl stress ,and also to mine the genetic resources of these two traditional cereal crops with strong resisitance to abiotic-stresses , the relative growth rate of seedings ,roots and leaves ,as well as some key physiological figures closely related to salt stress re-sponses were examined in hulless barley and barley ,which were planted under different treating periods of 1 .0%or 0 .5%NaCl .The result indicated that ,the germination rate of hulless barley seeds is significantly higher than that of barley under the same stress condition triggered by NaCl .As well as the relative growth rates of roots and leaves of hulless barley seedings are higher than those of barley .Subsequence data analysis of physiological phenotypes revealed that the content of MDA in leaves is significant lower in hulless barley ,while the content of free proline ,soluble sac-charides and the SOD activity in leaves are significantly higher in hulless barley under the same stress condition .As a conclusion ,we believe that the hulless barley harbors a better intracellular resistance to NaCl -stress than barley .