CAJ | 학술논문

目的：用蛋白质组学的方法，获得宫颈癌组织和非宫颈癌组织的差异蛋白表达图谱。寻找与宫颈癌发生、发展相关的特异蛋白，为探讨宫颈癌发病机制提供实验室依据。方法利用双向电泳、蛋白质串联质谱（MS／MS）分析宫颈癌的差异蛋白表达图谱，并于 NCBI 数据库中检索明确蛋白身份。结果经比对分析差异蛋白表达图谱及数据库检索，初步鉴定了12种蛋白，分别为：膜联蛋白 V 、膜联蛋白 I 、膜联蛋白 A1、膜联蛋白 A4、锰超氧化物歧化酶、转羟乙醛酶、假定蛋白、核不均一性核糖核蛋白 H2、载脂蛋白 A‐I 、热休克蛋白、L1蛋白、复杂的 T‐蛋白亚基伽玛亚型。结论这些蛋白可能与宫颈癌的发生、发展有关。
목적：용단백질조학적방법，획득궁경암조직화비궁경암조직적차이단백표체도보。심조여궁경암발생、발전상관적특이단백，위탐토궁경암발병궤제제공실험실의거。방법이용쌍향전영、단백질천련질보（MS／MS）분석궁경암적차이단백표체도보，병우 NCBI 수거고중검색명학단백신빈。결과경비대분석차이단백표체도보급수거고검색，초보감정료12충단백，분별위：막련단백 V 、막련단백 I 、막련단백 A1、막련단백 A4、맹초양화물기화매、전간을철매、가정단백、핵불균일성핵당핵단백 H2、재지단백 A‐I 、열휴극단백、L1단백、복잡적 T‐단백아기가마아형。결론저사단백가능여궁경암적발생、발전유관。
Objective To establish the two‐dimensional gel electrophoresis profiles with reproduc‐ibility and high resolution from cervical cancer tissue and non‐cervical cancer tissue .Find the differen‐tial expression of tumor‐associated proteins ,and provided the laboratory evidence of pathogenesis with cervical cancer genesis and development .Methods Gotten differences protein map by Two‐dimensional electrophoresis and mass spectrometry .Search in NCBI database according tandem mass spectrometry peptide fingerprint .Results Comparative analysis of screening out of differentially expressed protein spots ,initially identified 12 kinds of protein by mass spectrometry and database retrieval .They are as follows :Annexin V ,Annexin I ,Annexin A1 ,Annexin A4 ,Manganese superoxide dismutase ,tran‐sketolase , hypothetical protein , heterogeneous nuclear ribonucleoprotein H2 , Apolipoprotein A‐I , heat shock protein beta‐1 ,L1 protein ,T‐complex protein 1 subunit gamma isoform a .Conclusion The kinds of proteins may be related to the occurrence of cervical cancer to develop .