CAJ | 학술논문

目的：探讨痛泻要方治疗溃疡性结肠炎（ UC）大鼠的疗效及其机制。方法将实验动物分为3组：空白组、模型组、痛泻要方组，采用TNBS／乙醇溶液灌肠加束缚法制作UC肝郁脾虚证大鼠模型，光镜观察结肠组织病理形态变化，免疫组化法观察结肠组织过氧化物酶体增殖物激活受体（PPAR－γ）蛋白表达变化，并以大鼠胸腺指数、脾脏指数、结肠组织髓过氧化物酶（MPO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（TAOC）为观察指标。结果与空白组比较，模型组大鼠结肠组织损伤程度严重（P＜0．01），大鼠结肠组织 PPAR－γ蛋白表达下调（P＜0．01），脾脏和胸腺指数降低（P＜0．05），MPO和T－AOC活性降低（P＜0．05，P＜0．01），SOD活性升高（P＜0．05），MDA含量下降（P＜0．01）；与模型组比较，痛泻要方组的结肠损伤明显修复（P＜0．05），大鼠结肠组织 PPAR－γ蛋白表达上调（P＜0．01）；脾脏和胸腺指数升高，MPO 和 T－AOC 活性升高（P＜0．05）， SOD活性降低（P＜0．05），MDA含量增加（P＜0．05）。结论痛泻要方能够有效治疗实验性UC，其药效作用可能是通过提高结肠组织抗氧化能力和调节结肠组织紊乱的免疫功能以及提高结肠组织PPAR－γ的表达实现。
목적：탐토통사요방치료궤양성결장염（ UC）대서적료효급기궤제。방법장실험동물분위3조：공백조、모형조、통사요방조，채용TNBS／을순용액관장가속박법제작UC간욱비허증대서모형，광경관찰결장조직병리형태변화，면역조화법관찰결장조직과양화물매체증식물격활수체（PPAR－γ）단백표체변화，병이대서흉선지수、비장지수、결장조직수과양화물매（MPO）、병이철（MDA）、초양화물기화매（SOD）、총항양화능력（TAOC）위관찰지표。결과여공백조비교，모형조대서결장조직손상정도엄중（P＜0．01），대서결장조직 PPAR－γ단백표체하조（P＜0．01），비장화흉선지수강저（P＜0．05），MPO화T－AOC활성강저（P＜0．05，P＜0．01），SOD활성승고（P＜0．05），MDA함량하강（P＜0．01）；여모형조비교，통사요방조적결장손상명현수복（P＜0．05），대서결장조직 PPAR－γ단백표체상조（P＜0．01）；비장화흉선지수승고，MPO 화 T－AOC 활성승고（P＜0．05）， SOD활성강저（P＜0．05），MDA함량증가（P＜0．05）。결론통사요방능구유효치료실험성UC，기약효작용가능시통과제고결장조직항양화능력화조절결장조직문란적면역공능이급제고결장조직PPAR－γ적표체실현。
Objective To explore the curative effect and mechanism of Tongxie Yaofang on ulcerative colitis ( UC).Methods Meth-ods To divide the experimental animals into the blank, model and the Tongxie Yaofang groups, prepare the liver depression and spleen de-ficiency of UC models by means of the TNBS/ethanol enema and bound method.Colon tissue pathological morphology was observed under the light microscope, colonic tissue of peroxisome proliferator activated receptors ( PPAR-γ) protein expression changes were observed by immu-nohistochemistry, rat thymus index, spleen index, colon tissue pulp peroxidase (MPO), malondialdehyde (MDA), superoxide dismutase ( SOD) , total antioxidant capacity( TAOC) were all the observed indices.Results Compared with the blank group,colonic tissue injury was sever(P<0.01),colonic PPAR-γprotein expression was down regulated(P<0.01),spleen index and thymus index were reduced(P<0.05), MPO and TAOC activity were reduced (P<0.05,P<0.01),SOD activity was increased(P<0.05), MDA contents was decreased (P<0.01) in the model group.Compared with the model group, colon injury repair was obvious(P<0.05), colonic PPAR-γprotein expression was up regulated(P<0.01), spleen index and thymus index were raised, MPO and TAOC activity were raised(P<0.05), SOD activity was reduced (P<0.05), MDA contents was increased(P<0.05) in the Tongxie Yaofang group.Conclusions Tongxie Yaofang is effective in the treat-ment of experimental UC, which pharmacological actions may be by improving colon tissue oxidation resistance and regulating colonic tissue disorder of the immune function and increasing colonic PPAR-γexpression.