CAJ | 학술논문

研究了鲜枣中糖组成的高效液相色谱测定方法。样品经水超声提取，以Kromasil 100－5NH2（150×4.6mm）液相色谱柱分离，Waters 2414示差检测器进行检测。结果表明5种糖在0.1～10mg／mL范围内线性良好（r≥0.9992）。平均加标回收率在87.4％～96.2％之间；相对标准偏差 RSD （％）≤3.8％。该方法的最低检测限（％）范围为8.56×10－3～1.53×10－2。该方法简便、快速、灵敏，为鲜枣中糖组成分析提供了一种高效快速的测定方法。
연구료선조중당조성적고효액상색보측정방법。양품경수초성제취，이Kromasil 100－5NH2（150×4.6mm）액상색보주분리，Waters 2414시차검측기진행검측。결과표명5충당재0.1～10mg／mL범위내선성량호（r≥0.9992）。평균가표회수솔재87.4％～96.2％지간；상대표준편차 RSD （％）≤3.8％。해방법적최저검측한（％）범위위8.56×10－3～1.53×10－2。해방법간편、쾌속、령민，위선조중당조성분석제공료일충고효쾌속적측정방법。
Saccharides were extracted from samples with water and an ultrasonic extraction process. Sucrose ,glucose ,fructose ,maltose and lactose were separated on a Kromasil 100‐5NH2 column(150 × 4.6mm) ,analysed by Waters 2414 refractive index detector. The linear range was 0.1‐10mg/mL ,the cor‐relation coefficients was ≥0.9992. The average recoveries ranged from 87.4% to 96.2% ,and the relative standard deviation was ≤3.8%. The results indicate that the method is easier ,faster and more sensitive.