CAJ | 학술논문

目的：探讨大鼠脑桥呼吸调整中枢神经元 N-甲基-D-天氡氨酸（NMDA）受体及γ－氨基丁酸（GABA）受体对低氧呼吸反应的调节作用。方法通过显微注射技术向大鼠脑桥呼吸调整中枢内微量注射 NMDA受体阻断剂D-2-氨基-5-磷酸基戊酸（D-AP5）和GABA受体阻断剂荷包牡丹碱（BIC），观察其对低氧呼吸反应膈神经放电的影响。结果脑桥微量注射D-AP5后，急性低氧反应膈神经放电无显著性改变，但低氧后呼气时间延长的幅度及呼吸频率下降的幅度均减小（P均＜0．05），低氧后呼吸频率下降（PHFD）被削弱；脑桥微量注射BIC后，急性低氧反应膈神经放电频率升高的幅度及呼吸时间缩短的幅度均增大（P均＜0．05），PHFD无显著性改变。结论大鼠脑桥呼吸调整中枢神经元NMDA受体介导的突触传递主要影响 PHFD，GABA受体介导的突触传递主要影响急性低氧反应。
목적：탐토대서뇌교호흡조정중추신경원 N-갑기-D-천동안산（NMDA）수체급γ－안기정산（GABA）수체대저양호흡반응적조절작용。방법통과현미주사기술향대서뇌교호흡조정중추내미량주사 NMDA수체조단제D-2-안기-5-린산기무산（D-AP5）화GABA수체조단제하포모단감（BIC），관찰기대저양호흡반응격신경방전적영향。결과뇌교미량주사D-AP5후，급성저양반응격신경방전무현저성개변，단저양후호기시간연장적폭도급호흡빈솔하강적폭도균감소（P균＜0．05），저양후호흡빈솔하강（PHFD）피삭약；뇌교미량주사BIC후，급성저양반응격신경방전빈솔승고적폭도급호흡시간축단적폭도균증대（P균＜0．05），PHFD무현저성개변。결론대서뇌교호흡조정중추신경원NMDA수체개도적돌촉전체주요영향 PHFD，GABA수체개도적돌촉전체주요영향급성저양반응。
Objective To investigate the role of NMDA and GABA receptors located in pontine pneumotaxic center neurons in peripheral chemoreflex .Methods Experments were done on rats to observe the effects of NMDA receptor an-tagonist D-2-amino-5-phosphonovaleric acid ( D-AP5 ) and GABA receptor antagonist bicuculline ( BIC ) on the phrenic nerve discharges in peripheral chemoreflex by microinjecting the two antagonists into pontine .Results The post-hypoxic frequency decline ( PHFD ) was attenuated after microinjection of D-AP5 while the acute hypoxia response was not in-flunced.The rapid increase in respiratory frequency and the parallel shortening of expiratory time were enhanced after mi -croinjection of BIC , while PHFD was not influenced .Conculsion Neurotransmissions mediated by NMDA and GABA re-ceptors mainly modulate PHFD and the acute hypoxia response respectively .