CAJ | 학술논문

目的：建立基于分光光度法测定非那西丁与扑热息痛含量的肝脏储备功能评估方法。方法筛选分光光度测定的显色体系，确定最大吸收波长，分析不同因素对显色体系的影响，优化非那西丁与扑热息痛水解的最佳条件，最后对技术体系进行应用性验证。结果建立了利用分光光度测定样品中非那西丁和扑热息痛含量的技术体系，即样品加入3 mol／L盐酸水解30 min，加入0．02％1，2-萘醌-4-磺酸钠、1％十六烷基三甲基溴化铵及2％NaOH（或3％Na2CO3）（比例为1∶6∶1∶2或3），分别于500 nm和570 nm下测定吸光值，计算各自浓度。该技术测定血浆样品中非那西丁与扑热息痛时的分辨率和重复性与高效液相色谱法相当。结论利用分光光度技术测定血液中非那西丁与扑热息痛含量，二者比值具有评估肝脏储备功能价值。
목적：건립기우분광광도법측정비나서정여복열식통함량적간장저비공능평고방법。방법사선분광광도측정적현색체계，학정최대흡수파장，분석불동인소대현색체계적영향，우화비나서정여복열식통수해적최가조건，최후대기술체계진행응용성험증。결과건립료이용분광광도측정양품중비나서정화복열식통함량적기술체계，즉양품가입3 mol／L염산수해30 min，가입0．02％1，2-내곤-4-광산납、1％십륙완기삼갑기추화안급2％NaOH（혹3％Na2CO3）（비례위1∶6∶1∶2혹3），분별우500 nm화570 nm하측정흡광치，계산각자농도。해기술측정혈장양품중비나서정여복열식통시적분변솔화중복성여고효액상색보법상당。결론이용분광광도기술측정혈액중비나서정여복열식통함량，이자비치구유평고간장저비공능개치。
Objective To establish a liver reserve function assessment method based on detection of phenacetin and paracetamol by spectrophotometry .Methods To screen the spectrophotometric color system , determine the maximum ab-sorption wavelength , analyze the influence of color system in different factors , optimize optimal conditions that phenacetin and paracetamol hydrolyze , and then verify the application of this technology about the established system .Results The technology system detected phenacetin and paracetamol of blood samples using spectrophotometry was established , in which 3 mol/L hydrochloric acid was added to samples to hydrolyze for 30 min, then added 0.02%1,2-naphthoquinone-4-sulfon-ate, 1%sixteen alkyl bromide and 2%sodium hydroxide (or 3%sodium carbonate) (ratio of 1∶6∶1∶2 or 3), and then the absorbance was measured under 500 nm and 570 nm to calculate each concentration .Resolution and repeatability of technology used for determining phenacetin and paracetamol of plasma samples were comparable with HPLC .Conclusion Ratio of phenacetin and paracetamol under established spectrophotometric system could assess liver reserve function .