CAJ | 학술논문

目的：探讨不同保存条件和时间对丙型肝炎病毒（HCV）RNA稳定性的影响。方法将22份血浆样本置于－20℃保存4周，4℃保存12 d ，室温（20～25℃）保存7 d ，反复冻融5次，采用荧光定量聚合酶链反应测定HCV RNA水平，考察 HCV RNA稳定性。结果样本在－20℃保存4周 HCV RNA平均下降幅度小于或等于0．14 lg ，±95％ CI≤0．20 lg ；4℃保存12 d HCV RNA平均下降幅度小于或等于0．39 lg ，4℃保存12 d时95％CI下限为－0．50 lg ，＜12 d时±95％ CI≤0．24 lg ；室温保存7 d HCV RNA平均下降幅度小于或等于0．40 lg ，室温保存7 d处理组95％ CI下限为－0．53 lg ，＜7 d时±95％ CI≤0．29 lg ；反复冻融5次 HCV RNA平均下降幅度小于或等于0．10 lg ，±95％ C I≤0．15 lg。结论血浆样本在－20℃保存4周、4℃保存9 d、室温保存5 d和反复冻融5次时，对HCV RNA水平影响不明显，但4℃保存12 d或室温保存7 d后，RNA水平显著降低，所以应避免在这种条件和时间下保存和运输血浆。
목적：탐토불동보존조건화시간대병형간염병독（HCV）RNA은정성적영향。방법장22빈혈장양본치우－20℃보존4주，4℃보존12 d ，실온（20～25℃）보존7 d ，반복동융5차，채용형광정량취합매련반응측정HCV RNA수평，고찰 HCV RNA은정성。결과양본재－20℃보존4주 HCV RNA평균하강폭도소우혹등우0．14 lg ，±95％ CI≤0．20 lg ；4℃보존12 d HCV RNA평균하강폭도소우혹등우0．39 lg ，4℃보존12 d시95％CI하한위－0．50 lg ，＜12 d시±95％ CI≤0．24 lg ；실온보존7 d HCV RNA평균하강폭도소우혹등우0．40 lg ，실온보존7 d처리조95％ CI하한위－0．53 lg ，＜7 d시±95％ CI≤0．29 lg ；반복동융5차 HCV RNA평균하강폭도소우혹등우0．10 lg ，±95％ C I≤0．15 lg。결론혈장양본재－20℃보존4주、4℃보존9 d、실온보존5 d화반복동융5차시，대HCV RNA수평영향불명현，단4℃보존12 d혹실온보존7 d후，RNA수평현저강저，소이응피면재저충조건화시간하보존화운수혈장。
Objective To investigate effects of different storage conditions and time on stability of HCV RNA. Methods Totally 22 plasma samples were stored at different conditions as bellow :-20 ℃ for 4 weeks ;4 ℃for 12 days ;room temperature(20-25 ℃) for 7 days ;5 freeze‐thaw cycles. HCV RNA was determined by RT‐PCR to investigate the stability of it. Results The average decline of HCV RNA concentration was less than or equal to 0. 14 lg and ± 95% CI ≤0. 20 lg when samples were stored at -20 ℃ for 4 weeks. The average decline of HCV RNA concentration was less than or equal to 0. 39 lg and ± 95% CI ≥ -0. 50 lg when samples were stored at 4 ℃for 12 days. But ± 95% CI≤0. 24 lg when samples were stored at 4 ℃ less than 12 days. The average decline of HCV RNA concentration was less than or equal to 0. 40 lg and ± 95% CI≥ -0. 53 lg when samples were stored at room temperature for 7 days. But ± 95% CI≤0. 29 lg when samples were stored at room temperature less than 7 days. The average decline of HCV RNA concentration was less than or equal to 0. 10 lg and ± 95% CI≤0. 15 lg when samples were frozen and thawed for 5 times. Conclusion HCV RNA is stable in plasma samples stored at -20 ℃ for 4 weeks, 4 ℃ for 9 days, room temperature for 5 days or after 5 freeze‐thaw cycles. But HCV RNA concentration de‐clined dramatically when samples were stored at 4 ℃ for 12 days and room temperature for 7 days. The storage and transportation should be avoided in these conditions.